A miniaturized optical tomography platform for volumetric imaging of engineered living systems

Basit Öğe Kaydı
dc.authoridying, Guoliang/0000-0003-1781-6076
dc.authoridHassan, Shabir/0000-0002-5822-9508
dc.authoridMaharjan, Sushila/0000-0003-3957-3976
dc.authoridPolat, Adem/0000-0002-5662-4141
dc.contributor.authorPolat, Adem
dc.contributor.authorHassan, Shabir
dc.contributor.authorYildirim, Isa
dc.contributor.authorOliver, Luis Eduardo
dc.contributor.authorMostafaei, Maryam
dc.contributor.authorKumar, Siddharth
dc.contributor.authorMaharjan, Sushila
dc.date.accessioned2025-01-06T17:45:05Z
dc.date.available2025-01-06T17:45:05Z
dc.date.issued2019
dc.description.abstractVolumetric optical microscopy approaches that enable acquisition of three-dimensional (3D) information from a biological sample are attractive for numerous non-invasive imaging applications. The unprecedented structural details that these techniques provide have helped in our understanding of different aspects of architecture of cells, tissues, and organ systems as they occur in their natural states. Nonetheless, the instrumentation for most of these techniques is sophisticated, bulky, and costly, and is less affordable to most laboratory settings. Several miniature imagers based on webcams or low-cost sensors featuring easy assembly have been reported, for in situ imaging of biological structures at low costs. However, they have not been able to achieve the ability of 3D imaging throughout the entire volumes for spatiotemporal analyses of the structural changes in these specimens. Here we present a miniaturized optical tomography (mini-Opto) platform for lowcost, volumetric characterization of engineered living systems through hardware optimizations as well as applications of an optimized algebraic algorithm for image reconstruction.
dc.description.sponsorshipNational Institutes of Health [K99CA201603, R21EB025270, R21EB026175]; New England Anti-Vivisection Society (NEAVS); TUBITAK, the Scientific and Research Council of Turkey [1059B141600590]; Swiss National Science Foundation (SNSF) [P2ZHP3_161965, P300PA_177867]; National Natural Science Foundation of China [81503025]; NEAVS; American Fund for Alternatives to Animal Research (AFAAR); Natural and Science Foundation of Hubei Province, China [2014CFB778]; Swiss National Science Foundation (SNF) [P300PA_177867, P2ZHP3_161965] Funding Source: Swiss National Science Foundation (SNF)
dc.description.sponsorshipThis work was supported by the National Institutes of Health (K99CA201603, R21EB025270, R21EB026175) and the New England Anti-Vivisection Society (NEAVS). A.P. was supported by TUBITAK, the Scientific and Research Council of Turkey, under the grant 1059B141600590. S.H. acknowledges the financial support from the Swiss National Science Foundation (SNSF) under grant No. P2ZHP3_161965 and P300PA_177867. X.C. was supported by the National Natural Science Foundation of China (81503025). S.M. acknowledges NEAVS and the American Fund for Alternatives to Animal Research (AFAAR) for Postdoctoral Fellowship. G.L.Y. acknowledges Natural and Science Foundation of Hubei Province, China (2014CFB778).
dc.identifier.doi10.1039/c8lc01190g
dc.identifier.endpage561
dc.identifier.issn1473-0197
dc.identifier.issn1473-0189
dc.identifier.issue4
dc.identifier.pmid30657153
dc.identifier.scopus2-s2.0-85061358352
dc.identifier.scopusqualityQ1
dc.identifier.startpage550
dc.identifier.urihttps://doi.org/10.1039/c8lc01190g
dc.identifier.urihttps://hdl.handle.net/20.500.14669/3293
dc.identifier.volume19
dc.identifier.wosWOS:000459726400015
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofLab on A Chip
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectOn-A-Chıp
dc.subjectMultıcellular Spheroıds
dc.subjectOrganoıd Cultures
dc.subjectMını-Mıcroscope
dc.subjectCell Spheroıds
dc.subjectReconstructıon
dc.subjectCancer
dc.subjectVascularızatıon
dc.subjectBıosensor
dc.subjectNetworks
dc.titleA miniaturized optical tomography platform for volumetric imaging of engineered living systems
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu