Aptamer decorated PDA@magnetic silica microparticles for bacteria purification

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dc.authoridKIBAR, GUNES/0000-0002-2586-6770
dc.authoridozalp, cengiz/0000-0002-7659-5990
dc.authoridCetin, Barbaros/0000-0001-9824-4000
dc.authoridDURSUN, ALI DOGAN/0000-0001-9056-0025
dc.contributor.authorKavruk, Murat
dc.contributor.authorBabaie, Zahra
dc.contributor.authorKibar, Gunes
dc.contributor.authorCetin, Barbaros
dc.contributor.authorYesilkaya, Hasan
dc.contributor.authorAmrani, Yassine
dc.contributor.authorDursun, Ali Dogan
dc.date.accessioned2025-01-06T17:36:41Z
dc.date.available2025-01-06T17:36:41Z
dc.date.issued2024
dc.description.abstractOne significant constraint in the advancement of biosensors is the signal-to-noise ratio, which is adversely affected by the presence of interfering factors such as blood in the sample matrix. In the present investigation, a specific aptamer binding was chosen for its affinity, while exhibiting no binding affinity towards non-target bacterial cells. This selective binding property was leveraged to facilitate the production of magnetic microparticles decorated with aptamers. A novel assay was developed to effectively isolate S. pneumoniae from PBS or directly from blood samples using an aptamer with an affinity constant of 72.8 nM. The capture experiments demonstrated efficiencies up to 87% and 66% are achievable for isolating spiked S. pneumoniae in 1 mL PBS and blood samples, respectively.
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1007/s00604-024-06322-3
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue5
dc.identifier.pmid38652174
dc.identifier.scopus2-s2.0-85191068538
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-024-06322-3
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1956
dc.identifier.volume191
dc.identifier.wosWOS:001207219900003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectBiosensor
dc.subjectAptamer
dc.subjectAptasensor
dc.subjectSELEX
dc.subjectSuperparamagnetic
dc.subjectMicrobeads
dc.titleAptamer decorated PDA@magnetic silica microparticles for bacteria purification
dc.typeArticle

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