Aptamer decorated PDA@magnetic silica microparticles for bacteria purification
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Wien
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
One significant constraint in the advancement of biosensors is the signal-to-noise ratio, which is adversely affected by the presence of interfering factors such as blood in the sample matrix. In the present investigation, a specific aptamer binding was chosen for its affinity, while exhibiting no binding affinity towards non-target bacterial cells. This selective binding property was leveraged to facilitate the production of magnetic microparticles decorated with aptamers. A novel assay was developed to effectively isolate S. pneumoniae from PBS or directly from blood samples using an aptamer with an affinity constant of 72.8 nM. The capture experiments demonstrated efficiencies up to 87% and 66% are achievable for isolating spiked S. pneumoniae in 1 mL PBS and blood samples, respectively.
Açıklama
Anahtar Kelimeler
Biosensor, Aptamer, Aptasensor, SELEX, Superparamagnetic, Microbeads
Kaynak
Microchimica Acta
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
191
Sayı
5
