Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation

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dc.authoridKIBAR, GUNES/0000-0002-2586-6770
dc.authoridCetin, Barbaros/0000-0001-9824-4000
dc.authoridDoganay, Serkan/0000-0002-3237-693X
dc.contributor.authorKibar, Gunes
dc.contributor.authorSariarslan, Busra
dc.contributor.authorDoganay, Serkan
dc.contributor.authorYildiz, Gokay
dc.contributor.authorUsta, O. Berk
dc.contributor.authorCetin, Barbaros
dc.date.accessioned2025-01-06T17:45:10Z
dc.date.available2025-01-06T17:45:10Z
dc.date.issued2024
dc.description.abstractThis study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.
dc.description.sponsorshipT?rkiye Bilimsel ve Teknolojik Arastirma Kurumu [TUBITAK-2219, 1059B191801017]; Scientific and Technological Research Council of Turkiye; Turkish Fulbright Commission; Fulbright Postdoctoral Scholarship
dc.description.sponsorshipThis study is financially supported by the Scientific and Technological Research Council of Turkiye under grant no. TEYDEB-1170524. We also acknowledge the Turkish Fulbright Commission for a Fulbright Postdoctoral Scholarship and the Scientific and Technological Research Council of Turkiye (TUBITAK-2219 Award#1059B191801017) for Dr. G.K.
dc.identifier.doi10.1021/acs.analchem.3c04412
dc.identifier.endpage1992
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.issue5
dc.identifier.pmid38254336
dc.identifier.scopus2-s2.0-85184871119
dc.identifier.scopusqualityQ1
dc.identifier.startpage1985
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.3c04412
dc.identifier.urihttps://hdl.handle.net/20.500.14669/3338
dc.identifier.volume96
dc.identifier.wosWOS:001158550200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAnalytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectWhole-Blood
dc.subjectExtractıon Kıts
dc.subjectPurıfıcatıon
dc.subjectSılıca
dc.subjectAdsorptıon
dc.subjectSeparatıon
dc.subjectBehavıors
dc.subjectPolymer
dc.subjectBeads
dc.subjectNanopartıcles
dc.titleNovel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation
dc.typeArticle

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