Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Amer Chemical Soc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.
Açıklama
Anahtar Kelimeler
Whole-Blood, Extractıon Kıts, Purıfıcatıon, Sılıca, Adsorptıon, Separatıon, Behavıors, Polymer, Beads, Nanopartıcles
Kaynak
Analytical Chemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
96
Sayı
5
