AFB1 recognition from liver tissue via AFB1 imprinted magnetic nanoparticles
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Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Aflatoxins (AFs) are produced mainly by Aspergillus flavus and Aspergillus parasiticus and aflatoxin B1 (AFB1) is one of the most toxic aflatoxins with its carcinogenic property. AFB1 recognition from samples is very important and PHEMA based AFB1 imprinted magnetic nanoparticles (magAFB1-MIPs) were synthesized for the selective AFB1 recognition from liver tissue. The AFB1-MIPs were synthesized in different mole ratios and NIPs were synthesized for control. Characterization studies of magAFB1-MIPs and NIPs were carried out by swelling tests, surface area measurements, scanning electron microscopy and particle size analysis. The surface area was found as 117 m2/g and the size of the nanoparticles were found as 483 nm in diameter. The percentage yield of polymerization was calculated as 98 % and the template (AFB1) removal ratio from the magAFB1-MIPs was calculated as 91 %. The maximum adsorbtion capacities were calculated as 427.57 ng g-1 for magAFB1-MIPs and 44.6 ng g-1 for magNIPs. Selectivity tests showed that magAFB1-MIPs adsorb AFB1 1.74, 4.40, 2.46 times selective than that of AFB2, AFG1 and AFG2 molecules, respectively. AFB1 removal amount from AFB1 spiked liver tissue was satisfactory and recorded as 10.4 ng g-1 and 54.8 ng g-1 for 2 ng g-1 and 10 ng g-1 spiked liver tissue samples, respectively. AFB1 adsorption amount decrease was found negligible for 10 consecutive adsorption-desorption repeats in reusability study.
Açıklama
Anahtar Kelimeler
Mycotoxin detection, Aflatoxin B1, Molecular imprinting, Magnetic nanoparticles
Kaynak
Journal of Chromatography B-Analytical Technologies in The Biomedical and Life Sciences
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
1210
