Preparation of molecularly imprinted bilayer cryogel columns for selective protein depletion

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dc.authoridZenger, Okan/0000-0002-5669-0325
dc.authoridBaydemir Pesint, Gozde/0000-0001-8668-8296
dc.contributor.authorZenger, Okan
dc.contributor.authorPesint, Gozde Baydemir
dc.date.accessioned2025-01-06T17:43:16Z
dc.date.available2025-01-06T17:43:16Z
dc.date.issued2022
dc.description.abstractIn this study, bilayer-imprinted cryogels were synthesized for depletion of human serum albumin (HSA) from human serum. For this purpose, a monolayer HSA-imprinted cryogel with 8% monomer concentration was synthesized and second imprinting process was performed using a polymeric solution with 16% monomer concentration in presence of HSA, in the supermacroporous structure of first layer. Thereby, bilayer HSAimprinted cryogel column (HSA-BLIP) with enhanced surface area was obtained. Bilayer non-imprinted polymer (BL-NIP) and monolayer HSA-imprinted polymers with monomer concentrations of 8% and 16% (HSAMIP(8%) and HSA-MIP16%, respectively) were synthesized as the control group. Synthesized columns were characterized by swelling tests, scanning electron microscopy (SEM), multipoint Brunauer-Emmett-Teller (BET) surface area measurement and micro computed tomography (Micro-CT) analysis. Surface areas of HSA-BLIP, BL NIP, HSA-MIP8% and HSA-MIP(16% )were found to be 39 m(2)/g, 37 m(2)/g, 21 m(2)/g and 12 m(2)/g, respectively. Maximum HSA adsorption was calculated as 106.8 mg/g for HSA-BLIP and 5.14 mg/g for BL-NIP. Selectivity studies were conducted in presence of competitor molecules, human hemoglobin (Hb) and immunoglobulin G (IgG). Finally, selective depletion of HSA directly from human serum was studied. In addition, it was demonstrated that HSA-BLIPs can be used up to 10 times without significant decrease in selective adsorption capacity.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Adana Alparslan Turkes Science and Technology University [19103014]; completed in Nanotechnology Research and Application Laboratory in Adana Alparslan Turkes Science and Technology University/Cukurova Development Agency/Turkey [TR62/18URET/0032]
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Adana Alparslan Turkes Science and Technology University (Grant number: 19103014) and completed in Nanotechnology Research and Application Laboratory in Adana Alparslan Turkes Science and Technology University/Cukurova Development Agency/Turkey (TR62/18URET/0032).
dc.identifier.doi10.1016/j.procbio.2022.03.018
dc.identifier.endpage100
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85128982976
dc.identifier.scopusqualityQ1
dc.identifier.startpage90
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2022.03.018
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2578
dc.identifier.volume117
dc.identifier.wosWOS:000806790000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectHuman serum albumin
dc.subjectCryogels
dc.subjectBilayer-imprinting
dc.subjectMolecular Imprinting
dc.subjectProtein Depletion
dc.titlePreparation of molecularly imprinted bilayer cryogel columns for selective protein depletion
dc.typeArticle

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