Preparation of molecularly imprinted bilayer cryogel columns for selective protein depletion
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Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, bilayer-imprinted cryogels were synthesized for depletion of human serum albumin (HSA) from human serum. For this purpose, a monolayer HSA-imprinted cryogel with 8% monomer concentration was synthesized and second imprinting process was performed using a polymeric solution with 16% monomer concentration in presence of HSA, in the supermacroporous structure of first layer. Thereby, bilayer HSAimprinted cryogel column (HSA-BLIP) with enhanced surface area was obtained. Bilayer non-imprinted polymer (BL-NIP) and monolayer HSA-imprinted polymers with monomer concentrations of 8% and 16% (HSAMIP(8%) and HSA-MIP16%, respectively) were synthesized as the control group. Synthesized columns were characterized by swelling tests, scanning electron microscopy (SEM), multipoint Brunauer-Emmett-Teller (BET) surface area measurement and micro computed tomography (Micro-CT) analysis. Surface areas of HSA-BLIP, BL NIP, HSA-MIP8% and HSA-MIP(16% )were found to be 39 m(2)/g, 37 m(2)/g, 21 m(2)/g and 12 m(2)/g, respectively. Maximum HSA adsorption was calculated as 106.8 mg/g for HSA-BLIP and 5.14 mg/g for BL-NIP. Selectivity studies were conducted in presence of competitor molecules, human hemoglobin (Hb) and immunoglobulin G (IgG). Finally, selective depletion of HSA directly from human serum was studied. In addition, it was demonstrated that HSA-BLIPs can be used up to 10 times without significant decrease in selective adsorption capacity.
Açıklama
Anahtar Kelimeler
Human serum albumin, Cryogels, Bilayer-imprinting, Molecular Imprinting, Protein Depletion
Kaynak
Process Biochemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
117
