Enhanced laccase separation from fermentation medium using cryogel columns

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dc.authoridZenger, Okan/0000-0002-5669-0325
dc.authoridEREN YUNGEVIS, Burcu/0000-0001-6734-659X
dc.contributor.authorEren, Burcu
dc.contributor.authorZenger, Okan
dc.contributor.authorBasegmez, Hatice Imge Oktay
dc.contributor.authorPesint, Gozde Baydemir
dc.date.accessioned2025-01-06T17:37:45Z
dc.date.available2025-01-06T17:37:45Z
dc.date.issued2023
dc.description.abstractThe laccase enzyme family belongs to the oxidoreductase enzyme class and is one of the most commercially valuable enzymes that catalyzes the oxidation of one electron of a wide range of phenolic compounds. Separation and purification of laccases are crucial for industry since they play an important role in dye decolorization, biodegradation and food processing. Therefore, developing effective, high yielding and cost-effective methods for laccase production is vital. In this study, it was aimed to prepare cryogel columns for laccase purification following the bioproduction of laccase via Aspergillus niger. 2-hydroxyethyl methacrylate based cryogels were synthesized in the presence of 1-vinylimidazole as the affinity ligand and characterized by swelling tests, Bru-nauer-Emmett-Teller surface area measurement and scanning electron microscopy analysis. Surface area and water uptake ratio of cryogel columns were 35 m2/g and 93 %, respectively. The effect of pH, equilibrium laccase concentration, flow rate, interaction time and temperature on laccase adsorption were examined. The purifi-cation factor was calculated as 10.53 under optimum conditions and the enzyme recovery was found to be 86.7 % from fermentation medium. Current study revealed that laccase purification using cryogels following filtration of fermentation medium could be a promising candidate for industrial applications with eliminating the need for complex chromatographic steps.
dc.identifier.doi10.1016/j.jbiotec.2023.01.012
dc.identifier.endpage65
dc.identifier.issn0168-1656
dc.identifier.issn1873-4863
dc.identifier.pmid36708996
dc.identifier.scopus2-s2.0-85147542154
dc.identifier.scopusqualityQ1
dc.identifier.startpage58
dc.identifier.urihttps://doi.org/10.1016/j.jbiotec.2023.01.012
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2330
dc.identifier.volume364
dc.identifier.wosWOS:000930774300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectEnzyme
dc.subjectSeparation
dc.subjectAspergillus niger
dc.subjectAffinity chromatography
dc.titleEnhanced laccase separation from fermentation medium using cryogel columns
dc.typeArticle

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