Synthesis of Double-Layer Imprinted Polymers: BSA Depletion

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dc.contributor.authorZenger, Okan
dc.contributor.authorBaydemir Peşint, Gözde
dc.date.accessioned2025-01-06T17:29:57Z
dc.date.available2025-01-06T17:29:57Z
dc.date.issued2021
dc.description.abstractA sensitive, rapid, and cost-effective method for quantitative analysis of proteins (e.g., detection, purification, depletion) for a wide variety of purposes is required in a number of areas, such as immunodiagnostics and biotechnology. Double-layer imprinting technique, which is carried out via polymerization of polymer solution with higher monomer concentration, covering and filling the supermacroporous structure of a pre-synthesized cryogel column with a lower monomer concentration, thus improving the surface area and adsorption capacity of final product, is a brand new approach for the application of cryogels in molecular imprinting technology. Within the scope of this chapter, BSA is selected as a model protein for the application of double-layer imprinting protocol. In this chapter, synthesis of double-layer BSA-imprinted and non-imprinted cryogel columns (BSA-DLIP and DLNIP, respectively) are described. In addition, characterization of synthesized columns and BSA depletion studies from aqueous solutions are described in detail, as well as selectivity of BSA-DLIPs for BSA, against competitors. © 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
dc.identifier.doi10.1007/978-1-0716-1629-1_6
dc.identifier.endpage83
dc.identifier.issn1064-3745
dc.identifier.pmid34410660
dc.identifier.scopus2-s2.0-85113650789
dc.identifier.scopusqualityQ4
dc.identifier.startpage71
dc.identifier.urihttps://doi.org/10.1007/978-1-0716-1629-1_6
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1420
dc.identifier.volume2359
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofMethods in Molecular Biology
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectAdvanced adsorption capacity
dc.subjectBovine serum albumin
dc.subjectCryogel
dc.subjectDepletion
dc.subjectDouble-layer imprinted polymer
dc.subjectHigh-abundant proteins
dc.subjectMolecular imprinting technique
dc.titleSynthesis of Double-Layer Imprinted Polymers: BSA Depletion
dc.typeBook Chapter

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