Synthesis of Double-Layer Imprinted Polymers: BSA Depletion
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Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Humana Press Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A sensitive, rapid, and cost-effective method for quantitative analysis of proteins (e.g., detection, purification, depletion) for a wide variety of purposes is required in a number of areas, such as immunodiagnostics and biotechnology. Double-layer imprinting technique, which is carried out via polymerization of polymer solution with higher monomer concentration, covering and filling the supermacroporous structure of a pre-synthesized cryogel column with a lower monomer concentration, thus improving the surface area and adsorption capacity of final product, is a brand new approach for the application of cryogels in molecular imprinting technology. Within the scope of this chapter, BSA is selected as a model protein for the application of double-layer imprinting protocol. In this chapter, synthesis of double-layer BSA-imprinted and non-imprinted cryogel columns (BSA-DLIP and DLNIP, respectively) are described. In addition, characterization of synthesized columns and BSA depletion studies from aqueous solutions are described in detail, as well as selectivity of BSA-DLIPs for BSA, against competitors. © 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Açıklama
Anahtar Kelimeler
Advanced adsorption capacity, Bovine serum albumin, Cryogel, Depletion, Double-layer imprinted polymer, High-abundant proteins, Molecular imprinting technique
Kaynak
Methods in Molecular Biology
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
2359
