L-proline determination by molecularly imprinted nanoparticles: A potential nanoscale tool for the diagnosis of metabolic disorders

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dc.authoridBaydemir Pesint, Gozde/0000-0001-8668-8296
dc.contributor.authorNergiz, Mustafa
dc.contributor.authorZenger, Okan
dc.contributor.authorPesint, Gozde Baydemir
dc.date.accessioned2025-01-06T17:43:46Z
dc.date.available2025-01-06T17:43:46Z
dc.date.issued2024
dc.description.abstractDetecting and quantifying amino acids is vital in biochemical analyses, especially for diagnosing metabolic disorders. L-proline, among these amino acids, holds significant relevance for various metabolic disorders in living organisms, particularly in humans. hyperprolinemia arises when ineffective breakdown of L-proline occurs due to enzyme deficiencies, leading to its accumulation in the body and underscoring the need for precise monitoring. To address this challenge, molecular imprinting offers a reliable single-step technique for detecting target molecules like proteins, peptides, amino acids, or ions with high selectivity. Moreover, nanoparticles, with significant surface area-to-volume ratios, enable high-level mass transfer and binding kinetics, making them ideal for nano-scale sensitive applications. In this study, 2-hydroxyethyl methacrylate-based molecularly imprinted nanoparticles were synthesized via mini-emulsion polymerization, combining the advantages of molecular imprinting technique and nanoparticles for the specific recognition of L-proline, and were well-characterized by Scanning Electron Microscopy, zeta-sizer particle size analysis, and Fourier Transform Infrared Spectroscopy. Based on zeta-sizer analysis, the estimated diameters of L-proline-imprinted and non-imprinted nanoparticles (Pro-MIPs and NIPs) were determined to be approximately 27.51 nm and 20.66 nm, respectively. The adsorption of L-proline onto nanoparticles from aqueous solutions was investigated in a batch system, and the maximum Lproline adsorption capacity was determined to be 26.58 mg/g for Pro-MIPs and 4.65 mg/g for and NIPs. The selectivity of Pro-MIPs was assessed using Liquid Chromatography-Tandem Mass Spectrometry, even in human serum and in the presence of competing molecules (L-histidine and L-phenylalanine). Additionally, Pro-MIPs maintained their adsorption capacity through up to 10 adsorption-desorption cycles without significant decrease.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Adana Alparslan Turkes Science and Technology University [21303015]
dc.description.sponsorshipThe content of this article is based on the Master's thesis titled Designing of L-Proline specific nanoparticles for L-Proline detection from human serum, authored by Mustafa Nergiz, which was funded by the Scientific Research Projects Coordination Unit of Adana Alparslan Turkes Science and Technology University (Grant number: 21303015).
dc.identifier.doi10.1016/j.chroma.2024.465106
dc.identifier.issn0021-9673
dc.identifier.issn1873-3778
dc.identifier.pmid38917678
dc.identifier.scopus2-s2.0-85196761655
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.chroma.2024.465106
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2781
dc.identifier.volume1730
dc.identifier.wosWOS:001260277800001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Chromatography A
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectNanomaterials
dc.subjectMolecularly imprinted polymers
dc.subjectBiomarker detection
dc.subjectL-proline
dc.subjectHyperprolinemia
dc.titleL-proline determination by molecularly imprinted nanoparticles: A potential nanoscale tool for the diagnosis of metabolic disorders
dc.typeArticle

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