Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines

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dc.authoridOZDAS, Sibel/0000-0003-4610-2785
dc.contributor.authorOzdas, Sibel
dc.contributor.authorOzdas, Talih
dc.date.accessioned2025-01-06T17:38:19Z
dc.date.available2025-01-06T17:38:19Z
dc.date.issued2018
dc.description2nd International Mediterranean Science and Engineering Congress (IMSEC) -- OCT 25-27, 2017 -- Adana, TURKEY
dc.description.abstractHead and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. Current approaches for the treatment of HNSCC are not sufficient to increase the patient survival or to reduce the high recurrence rate. Consequently, there is a need to explore the molecular characteristics of this cancer in order to discover potential therapeutic target molecules. The overexpression of chromosome region maintenance 1 (Crm1), responsible for the transport of different classes of macromolecules from the nuclear membrane to the cytoplasm, in various cancer cells has made it an attractive target molecule in cancer research. It has been reported that transcription factors, which are the target cargo proteins of Crm1, have critical roles in regulating intracellular processes via their expression levels and functions, which in turn are regulated by the cell cycle and signaling proteins. Previous findings show that head and neck cancer cells overexpress Crm1 and that these cells become highly dependent on Crm1 function. The results of this study show that after decreasing Crm1 expression levels in HNSCC cells through either treatment with specific Crm1 RNA interference (siRNA) or the selective Crm1 inhibitor leptomycin B (LMB), cell viability, proliferation, migration, and wound-healing abilities decreased, suppressing tumorigenic properties through the induction of apoptosis. Crm1 is a powerful diagnostic biomarker because of its central role in cancerogenesis, and it has a high potential for the development of targeted Crm1 molecules or synthetic agents, such as LMB, as well as for the improvement of the clinical features in head and neck cancer.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114S948]
dc.description.sponsorshipThis study was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No. 114S948) and presented at the 2nd International Mediterranean Science and Engineering Congress, Adana, Turkey, 2017.
dc.identifier.doi10.3906/biy-1711-8
dc.identifier.endpage143
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue2
dc.identifier.pmid30814875
dc.identifier.scopus2-s2.0-85046031159
dc.identifier.scopusqualityQ1
dc.identifier.startpage132
dc.identifier.trdizinid349098
dc.identifier.urihttps://doi.org/10.3906/biy-1711-8
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/349098
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2542
dc.identifier.volume42
dc.identifier.wosWOS:000433481900004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectHead and neck cancer
dc.subjectchromosome region maintenance 1
dc.subjectmetastasis
dc.subjectRNA interference
dc.subjectleptomycin B
dc.titleCrm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines
dc.typeConference Object

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