Neopterin imprinted nanofilm decorated SPR sensors: Sensitive neopterin detection from human serum

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dc.authoridBaydemir Pesint, Gozde/0000-0001-8668-8296
dc.contributor.authorEren Yungevis, Burcu
dc.contributor.authorBaydemir Pesint, Gozde
dc.date.accessioned2025-01-06T17:43:47Z
dc.date.available2025-01-06T17:43:47Z
dc.date.issued2024
dc.description.abstractNeopterin (Np) is a valuable marker for monitoring infectious diseases and predicting prognosis. This study developed Np-imprinted surface plasmon resonance (SPR) sensors, specifically designed to detect Np in human serum. Characterization of the Np-imprinted (Np-MIP) and non-imprinted (NIP) SPR sensors was carried out using various techniques. The contact angle measurements revealed increased hydrophilicity of the gold sensor with the nanofilm covering, reducing the contact angles from 60.5 degrees to 45.87 degrees and 32.7 degrees for NIP and Np-MIP SPR sensors, respectively. The nanofilm thickness was determined as approximately 14 nm through AFM studies. Real-time Np detection was successfully achieved with the Np-MIP SPR sensors at different concentrations. The selectivity of Np-MIP was confirmed by testing against pterin and glucose, with Np recognition being 11 and 8.25 times higher than those of pterin and glucose, respectively. Np detection from human serum yielded a detection limit of 3 ng/mL. Furthermore, the synthesized Np-MIP SPR sensors demonstrated reusability over 10 cycles without any loss in Np binding capacity. These findings indicate that Np-MIP SPR sensors possess selective detection capabilities for Np in aqueous solutions and human serum.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Adana Alparslan Turkes Science and Technology University [21303001]; Nanotechnology Research and Application Laboratory in Adana Alparslan Turkes Science and Technology University/Cukurova Development Agency/Turkey [TR62/18UERET/0032]
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Adana Alparslan Turkes Science and Technology University (grant number: 21303001) and completed in the Nanotechnology Research and Application Laboratory in Adana Alparslan Turkes Science and Technology University/Cukurova Development Agency/Turkey (TR62/18UERET/0032).
dc.identifier.doi10.1007/s00289-024-05516-2
dc.identifier.endpage17252
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue18
dc.identifier.scopus2-s2.0-85204767867
dc.identifier.scopusqualityQ1
dc.identifier.startpage17233
dc.identifier.urihttps://doi.org/10.1007/s00289-024-05516-2
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2792
dc.identifier.volume81
dc.identifier.wosWOS:001319504100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectNeopterin
dc.subjectNanofilm
dc.subjectMolecular Imprinting
dc.subjectSurface plasmon resonance sensors
dc.titleNeopterin imprinted nanofilm decorated SPR sensors: Sensitive neopterin detection from human serum
dc.typeArticle

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