Neopterin imprinted nanofilm decorated SPR sensors: Sensitive neopterin detection from human serum
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Neopterin (Np) is a valuable marker for monitoring infectious diseases and predicting prognosis. This study developed Np-imprinted surface plasmon resonance (SPR) sensors, specifically designed to detect Np in human serum. Characterization of the Np-imprinted (Np-MIP) and non-imprinted (NIP) SPR sensors was carried out using various techniques. The contact angle measurements revealed increased hydrophilicity of the gold sensor with the nanofilm covering, reducing the contact angles from 60.5 degrees to 45.87 degrees and 32.7 degrees for NIP and Np-MIP SPR sensors, respectively. The nanofilm thickness was determined as approximately 14 nm through AFM studies. Real-time Np detection was successfully achieved with the Np-MIP SPR sensors at different concentrations. The selectivity of Np-MIP was confirmed by testing against pterin and glucose, with Np recognition being 11 and 8.25 times higher than those of pterin and glucose, respectively. Np detection from human serum yielded a detection limit of 3 ng/mL. Furthermore, the synthesized Np-MIP SPR sensors demonstrated reusability over 10 cycles without any loss in Np binding capacity. These findings indicate that Np-MIP SPR sensors possess selective detection capabilities for Np in aqueous solutions and human serum.
Açıklama
Anahtar Kelimeler
Neopterin, Nanofilm, Molecular Imprinting, Surface plasmon resonance sensors
Kaynak
Polymer Bulletin
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
81
Sayı
18
