Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning

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dc.authoridDenizci, Aziz Akin/0000-0002-0144-7918
dc.authoridPinar, Orkun/0000-0001-9133-3502
dc.authoridCakir Hatir, Pinar/0000-0002-3806-7118
dc.authoridGunduz, Oguzhan/0000-0002-9427-7574
dc.contributor.authorOzgoren, Tugba
dc.contributor.authorPinar, Orkun
dc.contributor.authorBozdag, Gulnihal
dc.contributor.authorDenizci, Aziz Akin
dc.contributor.authorGunduz, Oguzhan
dc.contributor.authorHatir, Pinar Cakir
dc.contributor.authorKazan, Dilek
dc.date.accessioned2025-01-06T17:36:31Z
dc.date.available2025-01-06T17:36:31Z
dc.date.issued2018
dc.description.abstractIn this study, poly(3-hydroxybutyrate) (PHB) production from a newly isolated obligate alkaliphilic Bacillus marmarensis DSM 21297 was investigated to evaluate the ability of obligate alkaliphilic strain to produce a biopolymer. Additionally, electrospun nanofibers from B. marmarensis PHB (Bm-PHB) were generated using Bm-PHB/polycaprolactone (PCL) blend to evaluate the applicability of Bm-PHB. According to the experimental results, the metabolic activity of B. marmarensis decreased the pH of the medium by generating H+ ions to initiate Bm-PHB production, which was achieved at pH below 9.0. Regarding medium components, the addition of MgSO4 center dot 7H(2)O and KH2PO4 to the medium containing 1% glucose enhanced the amount of Bm-PHB synthesis, and an approximately 60% increase in PHB concentration was obtained in the presence of mineral salts. Based on FTIR analysis, the chemical structures of Bm-PHB and commercial PHB were found to be highly similar. Additionally, the T-g and T-m values of Bm-PHB were determined to be 17.77 degrees C and 165.17 degrees C, respectively. Moreover, Bm-PHB/PCL composite scaffold was generated by electrospinning method that produced nanofibers between 150 and 400 nm in diameter, with an average of 250 nm. To our knowledge, this is the first report to produce PHB from an obligate alkaliphilic Bacillus strain and PHB scaffold. (C) 2018 Elsevier B.V. All rights reserved.
dc.description.sponsorshipMarmara University, Scientific Research Projects Committee [FEN-C-YLP-080715-03418]; Scientific and Technological Research Council of Turkey (TUBITAK) [1007, 115G079]
dc.description.sponsorshipThis work was supported by Marmara University, Scientific Research Projects Committee -[grant number FEN-C-YLP-080715-03418] and Scientific and Technological Research Council of Turkey (TUBITAK) with 1007 - Public Institutions Research Funding Program (KAMAG) [grant number 115G079].
dc.identifier.doi10.1016/j.ijbiomac.2018.08.014
dc.identifier.endpage991
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid30092306
dc.identifier.scopus2-s2.0-85051136779
dc.identifier.scopusqualityQ1
dc.identifier.startpage982
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2018.08.014
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1912
dc.identifier.volume119
dc.identifier.wosWOS:000447682100110
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectBacillus marmarensis
dc.subjectPoly(3-hydroxybutyrate)
dc.subjectComposite nanofiber scaffold
dc.titleAssessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning
dc.typeArticle

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