Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells

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dc.contributor.authorYurtsever, Merve Capkin
dc.contributor.authorAkdere, Ozge Ekin
dc.contributor.authorGumusderelioglu, Menemse
dc.date.accessioned2025-01-06T17:43:33Z
dc.date.available2025-01-06T17:43:33Z
dc.date.issued2022
dc.description.abstractChitosan has high biocompatibility, supports proliferation of many cells, and can be a good carrier for various growth factors. However, low attachment ratio and spheroid formation of several stem cell types on plain chitosan scaffolds/films is still a problem. In this study, it was aimed to obtain 3D scaffolds using medical grade chitosan (MC) with a high deacetylation degree (DD >= 92.6%) to overcome the spheroid formation of rat adipose tissue derived mesenchymal stem cells (rAdMSCs) on control chitosan (C, DD = 75%-85%) scaffolds. Genipin was used as a biological chemical crosslinker, and glycerol phosphate salt was used both as a pH adjusting agent and physical crosslinker. MTT and SEM analyses and live/dead staining indicated the increase in the attachment, cell viability, and proliferation of rAdMSCs on MC scaffolds with or without crosslinking when compared to the cells in spheroid formation on control scaffolds. Moreover, filamentous actin protein organization of rAdMSCs was found to be triggered on the crosslinked MC scaffolds. In conclusion, plain medical grade chitosan scaffolds with or without crosslinking prevented spheroid formation, supported the attachment, proliferation, and organization of rAdMSCs indicating that medical grade type of chitosan scaffolds with high DD can be a very good candidate as 3D carriers in stem cell cultivation.
dc.description.sponsorshipAdana Alparslan Turkes Science and Technology University, Scientific Research Projects Coordination Unit [16103012]
dc.description.sponsorshipThe authors received support from Adana Alparslan Turkes Science and Technology University, Scientific Research Projects Coordination Unit, Project Number 16103012.
dc.identifier.doi10.55730/1300-0152.2633
dc.identifier.endpage487
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue6
dc.identifier.pmid37529794
dc.identifier.scopus2-s2.0-85144259105
dc.identifier.scopusqualityQ1
dc.identifier.startpage475
dc.identifier.trdizinid1145885
dc.identifier.urihttps://doi.org/10.55730/1300-0152.2633
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1145885
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2711
dc.identifier.volume46
dc.identifier.wosWOS:000911317900005
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectMedical grade chitosan
dc.subjectgenipin
dc.subjectstem cell
dc.subjectscaffold
dc.titleScaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells
dc.typeArticle

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