Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR

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dc.contributor.authorKadiro?lu, Pinar
dc.contributor.authorKorel, Figen
dc.contributor.authorCeylan, Cagatay
dc.date.accessioned2025-01-06T17:30:10Z
dc.date.available2025-01-06T17:30:10Z
dc.date.issued2014
dc.description.abstractFour different bacterial DNA extraction strategies and two different qPCR probe chemistries were studied for detection of Stapylococcus aureus from white cheeses. Method employing trypsin treatment followed by a commercial kit application and TaqMan probe-based qPCR was the most sensitive one detecting higher counts than standards in naturally contaminated samples. © 2014 Elsevier B.V.
dc.description.sponsorshipİzmir Yüksek Teknoloji Enstitüsü, İYTE, (2010-IYTE-05)
dc.identifier.doi10.1016/j.mimet.2014.06.022
dc.identifier.endpage97
dc.identifier.issn0167-7012
dc.identifier.pmid25016130
dc.identifier.scopus2-s2.0-84905484243
dc.identifier.scopusqualityQ3
dc.identifier.startpage92
dc.identifier.urihttps://doi.org/10.1016/j.mimet.2014.06.022
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1486
dc.identifier.volume105
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Microbiological Methods
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectCheese
dc.subjectLNA
dc.subjectQPCR
dc.subjectStaphylococcus aureus
dc.subjectTaqMan
dc.titleQuantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR
dc.typeArticle

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