Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR
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Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Four different bacterial DNA extraction strategies and two different qPCR probe chemistries were studied for detection of Stapylococcus aureus from white cheeses. Method employing trypsin treatment followed by a commercial kit application and TaqMan probe-based qPCR was the most sensitive one detecting higher counts than standards in naturally contaminated samples. © 2014 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Cheese, LNA, QPCR, Staphylococcus aureus, TaqMan
Kaynak
Journal of Microbiological Methods
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
105
