Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72T Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis

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dc.authoridKazan, Dilek/0000-0002-0764-8876
dc.authoridPinar, Orkun/0000-0001-9133-3502
dc.contributor.authorAtakav, Yagmur
dc.contributor.authorPinar, Orkun
dc.contributor.authorKazan, Dilek
dc.date.accessioned2025-01-06T17:36:31Z
dc.date.available2025-01-06T17:36:31Z
dc.date.issued2021
dc.description.abstractThe novel extreme obligate alkaliphilic Bacillus marmarensis DSM 21297 is known to produce polyhydroxybutyrate (PHB). However, the detailed mechanism of PHB synthesis in B. marmarensis is still unknown. Here, we investigated which metabolic pathways and metabolic enzymes are responsible for PHB synthesis in order to understand the regulatory pathway and optimize PHB synthesis in B. marmarensis. In accordance with the fact that beta-galactosidase, 3-hydroxyacyl-CoA dehydrogenase, and Enoyl-CoA hydratase together with acyl-CoA dehydrogenase and lipase were annotated in B. marmarensis according to the RAST server, we used glucose, lactose, and olive oil to understand the preferred metabolic pathway for the PHB synthesis. It was found that B. marmarensis produces PHB from glucose, lactose, and olive oil. However, the highest PHB titer and the highest amount of PHB synthesized per dry cell mass (Y-P/X) were achieved in the presence of lactose, as compared to glucose and olive oil. Additionally, in the absence of peptone, the amount of PHB synthesized is reduced for each carbon source. Interestingly, none of the carbon sources studied yielded an efficient PHB synthesis, and supplementation of the medium with potassium ions did not enhance PHB synthesis. According to these experimental results and the presence of annotated metabolic enzymes based on the RAST server, PHB accumulation in the cells of B. marmarensis could be improved by the level of the expression of 3-hydroxybutyryl-CoA dehydrogenase (1.1.1.157), which increases the production of NADPH. Additionally, the accumulation of 3-hydroxyacyl-CoA could enhance the production of PHB in B. marmarensis in the presence of fatty acids. To our knowledge, this is the first report investigating the regulatory system involved in the control of PHB metabolism of B. marmarensis.
dc.description.sponsorshipMarmara University, Scientific Research Projects Committee [FEN-B-170419-0120]
dc.description.sponsorshipThis study was supported by Marmara University, Scientific Research Projects Committee [FEN-B-170419-0120].
dc.identifier.doi10.3390/microorganisms9020462
dc.identifier.issn2076-2607
dc.identifier.issue2
dc.identifier.pmid33672214
dc.identifier.scopus2-s2.0-85101247915
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/microorganisms9020462
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1913
dc.identifier.volume9
dc.identifier.wosWOS:000622809600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofMicroorganisms
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectBacillus marmarensis
dc.subjectpolyhydroxybutyrate (PHB)
dc.subjectlactose
dc.subjectalkaline adaptation
dc.titleInvestigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72T Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis
dc.typeArticle

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