Enhanced invertase binding from baker's yeast via cryogels included boronic acids

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dc.authoridPercin, Isik/0000-0002-9453-4462
dc.authoridEREN YUNGEVIS, Burcu/0000-0001-6734-659X
dc.contributor.authorPesint, Gozde Baydemir
dc.contributor.authorYungevis, Burcu Eren
dc.contributor.authorDemircelik, Isik Percin
dc.date.accessioned2025-01-06T17:43:20Z
dc.date.available2025-01-06T17:43:20Z
dc.date.issued2023
dc.description.abstractInvertase, an industrially significant glycoenzyme, was purified from baker's yeast using poly (2-Hydroxyethyl methacrylate) [PHema-Pba] cryogels functionalized with boronic acid. At subzero temperatures, PHema-Pba cryogels were synthesized and characterized using swelling tests, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The surface area of the PHema- Pba cryogels was 14 m(2)/g with a swelling ratio of 88.3% and macroporosity of 72%. The interconnected macropores of PHema-Pba cryogels were shown via scanning electron microscopy. Invertase binding capacity of PHema-Pba cryogel was evaluated by binding studies in different pH, temperature, and interaction time conditions and the maximum Invertase binding of PHema-Pba cryogel was found as 15.2 mg/g. and 23.7 fold Invertase purification was achieved from baker's yeast using PHema-Pba cryogels. The results show that PHema-Pba cryogels have high Invertase binding capacity and may be used as an alternative method for enzyme purification via boronate affinity systems. [GRAPHICS] .
dc.identifier.doi10.1007/s11274-023-03697-y
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue10
dc.identifier.pmid37528302
dc.identifier.scopus2-s2.0-85166045519
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11274-023-03697-y
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2629
dc.identifier.volume39
dc.identifier.wosWOS:001041358900002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofWorld Journal of Microbiology & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectInvertase
dc.subjectCryogel
dc.subjectEnzyme purification
dc.subjectBaker's yeast
dc.subjectSaccharomyces cerevisiae
dc.titleEnhanced invertase binding from baker's yeast via cryogels included boronic acids
dc.typeArticle

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