Propolis loaded and genipin-crosslinked PVA/chitosan membranes; characterization properties and cytocompatibility/genotoxicity response for wound dressing applications

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dc.authoridceylan, seda/0000-0002-1088-7886
dc.contributor.authorCeylan, Seda
dc.date.accessioned2025-01-06T17:44:05Z
dc.date.available2025-01-06T17:44:05Z
dc.date.issued2021
dc.description.abstractLoading propolis by a simple process using genipin as a crosslinking agent and fabrication of a novel PVA/ Chitosan-Propolis membrane scaffolds were reported for wound dressing applications. The research is focused on the effects of propolis on characterization properties of membrane such as chemical structure, surface morphology, degradation ratio, crystallinity, hydrophilicity, water uptake capacity, water vapour transmission rate and mechanical aspect. It was noticed that water uptake capacity and hydrophilicity properties of membrane considerably affected by the propolis. By addition of (0.50, % v/v) propolis, the contact angle of the PVA/Chitosan membrane was remarkably decreased from 86.29 degrees +/- 3 to 45 +/- 2 degrees. 3-(4,5-dimethylthiazoyl-2-yl)-2,5diphenylte-trazolium (MTT) bromide test and SEM were used to analyse the cytocompatibility of the membranes and morphology of cells on membrane. The propolis incorporated membrane showed cell proliferation rate 176 +/- 13%, 775 +/- 1%, and 853 +/- 23%, at 24 h, 27 h and 120 h, respectively. SEM images also supported the cell behaviour on membrane. DNA fragmentation was also investigated with genotoxicity test. The studies on the interactions between membranes and MEF cells revealed that the incorporation of propolis into membrane promoted cell proliferation. These overall results presented that propolis incorporated membranes could have potentially appealing application as scaffolds for wound healing applications. (c) 2021 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific Research Projects Unit of Adana Alparslan Turkes Science and Technology University [BAP-20103004]
dc.description.sponsorshipThis work was supported financially by the Scientific Research Projects Unit of Adana Alparslan Turkes Science and Technology University (BAP-20103004) and carried out in Bioengineering laboratories of this university. The authorwould like to express their special gratitude to research assistant M.Damla Ozdemir for her supporting in experimental procedure of cytocompatibility studies.
dc.identifier.doi10.1016/j.ijbiomac.2021.05.069
dc.identifier.endpage1206
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid33991555
dc.identifier.scopus2-s2.0-85105787800
dc.identifier.scopusqualityQ1
dc.identifier.startpage1196
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2021.05.069
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2924
dc.identifier.volume181
dc.identifier.wosWOS:000656916800010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectPropolis
dc.subjectPVA
dc.subjectChitosan
dc.subjectGenipin
dc.titlePropolis loaded and genipin-crosslinked PVA/chitosan membranes; characterization properties and cytocompatibility/genotoxicity response for wound dressing applications
dc.typeArticle

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