Spongy membranes for peroxidase purification from Brassica oleracea roots

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dc.authoridPercin, Isik/0000-0002-9453-4462
dc.authoridZenger, Okan/0000-0002-5669-0325
dc.authoridBaydemir Pesint, Gozde/0000-0001-8668-8296
dc.contributor.authorPesint, Gozde Baydemir
dc.contributor.authorZenger, Okan
dc.contributor.authorPercin, Isik
dc.contributor.authorDenizli, Adil
dc.date.accessioned2025-01-06T17:44:26Z
dc.date.available2025-01-06T17:44:26Z
dc.date.issued2021
dc.description.abstractIn this work, it was aimed to purify peroxidase from Brassica oleracea roots using newly synthesized cryogel membranes. 2-4-vinylphenylboronic acid (VPBA) containing 2-hydroxyethyl methacrylate (HEMA) [PHE-MABA] cryogel membranes were prepared successfully and swelling tests, scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, contact angle measurements, and Brauner-Emmet-Teller (BET) surface area analysis were done for characterization. Effect of experimental conditions on peroxidase binding capacity of PHEMABA cryogel membranes was determined by examining parameters such as pH, temperature and time with binding studies. Maximum peroxidase binding capacity of PHEMABA cryogel membranes was determined as 22.01 mg/g for 2 mg/mL equilibrium peroxidase concentration at pH 8.0. Finally, peroxidase was purified from Brassica oleracea roots with 85.38% yield and 18.38 purification fold. According to obtained re-sults, spongy PHEMABA cryogel membranes show high peroxidase binding capacity and could be suggested as a boronate affinity model system for enzyme purification.
dc.identifier.doi10.1016/j.procbio.2021.02.005
dc.identifier.endpage106
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85101353221
dc.identifier.scopusqualityQ1
dc.identifier.startpage98
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2021.02.005
dc.identifier.urihttps://hdl.handle.net/20.500.14669/3059
dc.identifier.volume103
dc.identifier.wosWOS:000632024900003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectSpongy membrane
dc.subject2-4-Vinylphenylboronic acid
dc.subjectPeroxidase
dc.subjectEnzyme purification
dc.subjectCryogel
dc.titleSpongy membranes for peroxidase purification from Brassica oleracea roots
dc.typeArticle

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