Spongy membranes for peroxidase purification from Brassica oleracea roots
[ X ]
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this work, it was aimed to purify peroxidase from Brassica oleracea roots using newly synthesized cryogel membranes. 2-4-vinylphenylboronic acid (VPBA) containing 2-hydroxyethyl methacrylate (HEMA) [PHE-MABA] cryogel membranes were prepared successfully and swelling tests, scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, contact angle measurements, and Brauner-Emmet-Teller (BET) surface area analysis were done for characterization. Effect of experimental conditions on peroxidase binding capacity of PHEMABA cryogel membranes was determined by examining parameters such as pH, temperature and time with binding studies. Maximum peroxidase binding capacity of PHEMABA cryogel membranes was determined as 22.01 mg/g for 2 mg/mL equilibrium peroxidase concentration at pH 8.0. Finally, peroxidase was purified from Brassica oleracea roots with 85.38% yield and 18.38 purification fold. According to obtained re-sults, spongy PHEMABA cryogel membranes show high peroxidase binding capacity and could be suggested as a boronate affinity model system for enzyme purification.
Açıklama
Anahtar Kelimeler
Spongy membrane, 2-4-Vinylphenylboronic acid, Peroxidase, Enzyme purification, Cryogel
Kaynak
Process Biochemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
103
