Mapping the Molecular Basis and Markers of Papillary Thyroid Carcinoma Progression and Metastasis Using Global Transcriptome and microRNA Profiling

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dc.authoridOZBEK, BUSRA/0000-0002-4221-0845
dc.contributor.authorAkyay, Ozlem Zeynep
dc.contributor.authorGov, Esra
dc.contributor.authorKenar, Halime
dc.contributor.authorArga, Kazim Yalcin
dc.contributor.authorSelek, Alev
dc.contributor.authorTarkun, Ilhan
dc.contributor.authorCanturk, Zeynep
dc.date.accessioned2025-01-06T17:43:52Z
dc.date.available2025-01-06T17:43:52Z
dc.date.issued2020
dc.description.abstractPapillary thyroid carcinoma (PTC) is the most common type of thyroid cancer (TC). In a subgroup of patients with PTC, the disease progresses to an invasive stage or in some cases to distant organ metastasis. At present, there is an unmet clinical and diagnostic need for early identification of patients with PTC who are at risk of disease progression or metastasis. In this study, we report several molecular leads and potential biomarker candidates of PTC metastasis for further translational research. The study design was based on comparisons of PTC in three different groups using cross-sectional sampling: Group 1, PTC localized to the thyroid (n = 20); Group 2, PTC with extrathyroidal progression (n = 22); and Group 3, PTC with distant organ metastasis (n = 20). Global transcriptome and microRNAs (miRNA) analyses were conducted using an initial screening set comprising nine formalin-fixed paraffin-embedded PTC samples obtained from three independent patients per study group. The findings were subsequently validated by quantitative real-time polymerase chain reaction (qRT-PCR) using the abovementioned independent patient sample set (n = 62). Comparative analyses of differentially expressed miRNAs showed that miR-193-3p, miR-182-5p, and miR-3607-3p were novel miRNAs associated with PTC metastasis. These potential miRNA biomarkers were associated with TC metastasis and miRNA-target gene associations, which may provide important clinicopathological information on metastasis. Our findings provide new molecular leads for further translational biomarker research, which could facilitate the identification of patients at risk of PTC disease progression or metastasis.
dc.description.sponsorshipKocaeli University Department of Scientific Research Projects [2015/034]
dc.description.sponsorshipThis study was sponsored by the Kocaeli University Department of Scientific Research Projects (2015/034).
dc.identifier.doi10.1089/omi.2019.0188
dc.identifier.endpage159
dc.identifier.issn1536-2310
dc.identifier.issn1557-8100
dc.identifier.issue3
dc.identifier.pmid32073999
dc.identifier.scopus2-s2.0-85082137860
dc.identifier.scopusqualityQ2
dc.identifier.startpage148
dc.identifier.urihttps://doi.org/10.1089/omi.2019.0188
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2841
dc.identifier.volume24
dc.identifier.wosWOS:000515386400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofOmics-A Journal of Integrative Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectpapillary thyroid carcinoma
dc.subjectmicroRNA
dc.subjectmRNA
dc.subjectmetastasis
dc.subjectbiomarkers
dc.subjectgenomics
dc.titleMapping the Molecular Basis and Markers of Papillary Thyroid Carcinoma Progression and Metastasis Using Global Transcriptome and microRNA Profiling
dc.typeArticle

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