Melissa officinalis essential oil loaded polycaprolactone membranes: evaluation of antimicrobial activities and cytocompatibility for tissue engineering applications

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dc.authoridceylan, seda/0000-0002-1088-7886
dc.authoridCapkin Yurtsever, Merve/0000-0001-7874-4016
dc.authoridIyigundogdu, Zeynep/0000-0003-2067-4822
dc.authoridPetek, Betul Sena/0009-0005-1025-5079
dc.contributor.authorIyigundogdu, Zeynep
dc.contributor.authorPetek, Betul Sena
dc.contributor.authorYurtsever, Merve Capkin
dc.contributor.authorCeylan, Seda
dc.date.accessioned2025-01-06T17:43:33Z
dc.date.available2025-01-06T17:43:33Z
dc.date.issued2023
dc.description.abstractAntimicrobial biomaterials play important role in tissue engineering applications to protect damaged tissue from infections. The aim of this study is producing antimicrobial polycaprolactone (PCL) membranes by using a plant based antimicrobial agent. Therefore,Melissa officinalisessential oil (MEO) was investigated against ten types of microorganisms and remarkable antimicrobial activity was demonstrated. PCL:MEO membranes were prepared by solvent casting method by mixing MEO into PCL in various ratios (PCL:0M, PCL:0.25M, PCL:0.5M, and PCL:1M w/w). Water contact angle measurements showed that hydrophilicity of the membranes increased with increasing concentrations of MEO from 103.44 degrees to 83.36 degrees for PCL:0M and PCL:1M, respectively. It was determined that there was an inverse relationship between the MEO concentration and the mechanical properties. Notable antioxidant activity of PCL/MEO membranes was exhibited by the inhibition percent of 2,2-diphenyl-1-picrylhydrazyl (DPPH) which was increased from 24.74% to 44.79% for PCL:0M and PCL:1M, respectively. The antimicrobial activity of MEO was also highly maintained in PCL membranes. For PCL/MEO membranes, at least 99.9% of microorganisms were inhibited. Cytocompatibility of the membranes were investigated by resazurin assay, scanning electron microscopy analysis and 4',6-diamidino-2-phenylindole (DAPI) staining. PCL:0.25M and PCL:0.5M membranes supported the viability of L929 cells more than 87% when compared to PCL:0M membranes on day 6. However, the viability of L929 cells on PCL:1M membranes was about 43% indicating significant decrease on cellular activity. In conclusion, PCL:0.25M and PCL:0.5M membranes with their high antimicrobial activity, acceptable mechanical properties and cytocompatible properties, they can be considered as an alternative biomaterial for tissue engineering applications.
dc.description.sponsorshipScientific Research Projects Unit of Adana Alparslan Turkes Science and Technology University [BAP- 21103012]; Scientific and Technological Research Council of Turkiye [TUBITAK 2209A/1919B012003944]
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Unit of Adana Alparslan Turkes Science and Technology University (BAP- 21103012) and The Scientific and Technological Research Council of Turkiye (TUBITAK 2209A/1919B012003944).
dc.identifier.doi10.1088/1748-605X/acfc9d
dc.identifier.issn1748-6041
dc.identifier.issn1748-605X
dc.identifier.issue6
dc.identifier.pmid37741274
dc.identifier.scopus2-s2.0-85175014046
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1088/1748-605X/acfc9d
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2713
dc.identifier.volume18
dc.identifier.wosWOS:001083946700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherIop Publishing Ltd
dc.relation.ispartofBiomedical Materials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectMelissa officinalis essential oil
dc.subjectpolycaprolactone
dc.subjectmembrane
dc.subjectantimicrobial activity
dc.subjectcytocompatibility
dc.subjectantioxidant activity
dc.titleMelissa officinalis essential oil loaded polycaprolactone membranes: evaluation of antimicrobial activities and cytocompatibility for tissue engineering applications
dc.typeArticle

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