Non-Detection of HCMV Total Genomic DNA in Human Glioma Cells Genome

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dc.authoridOzdemir Alkis, Meryem Damla/0000-0002-5728-8004
dc.contributor.authorGokturk, Dilek
dc.contributor.authorAlkis, M. Damla Ozdemir
dc.contributor.authorYilmaz, Dervis Mansuri
dc.date.accessioned2025-01-06T17:38:09Z
dc.date.available2025-01-06T17:38:09Z
dc.date.issued2024
dc.description.abstractAIM: To demonstrate if the human cytomegalovirus (HCMV) genome, that is involved in the pathogenesis of gliomas, is part of the genomic DNA of glioma cells or not. MATERIAL and METHODS: The study included U87MG glioblastoma cell culture and tumor samples from glioma patients. The genomic DNA of tumor samples and U87MG cells were extracted and real-time quantitative PCR was used to assess the presence of the human cytomegalovirus genomic DNA. RESULTS: Consequently, HCMV positivity was not detected in the tumor and cell line genomic DNA under the aforementioned experimental conditions. CONCLUSION: We found that the genomic DNA of all the samples was negative for HCMV genomic DNA. Thus, HCMV could not be detected in human glioma tumors and we put forward that HCMV genomic DNA was not incorporated into the genomic DNA of glioma cells. Thus, total viral DNA is not involved in the pathogenesis of glioma; however, small viral particles or specific genes might be incorporated into the genomic DNA of glioma cells, leading to cancer development. This prompts further studies for verification.
dc.description.sponsorshipScientific Research Projects Fund [MUHDBF.BYO.2014-5]
dc.description.sponsorshipThe Scientific Research Projects Fund (Project number. MUHDBF.BYO.2014-5) provided funding for this work. The Federation of European Biochemical Societies (FEBS) Journal Special Issue for the 41 st FEBS Congress published the work's abstract as an oral presentation. Authors express their gratitude to Assoc. Prof. Seda Ceylan for her assistance in the transportation of some biological samples.
dc.identifier.doi10.5137/1019-5149.JTN.42717-22.1
dc.identifier.endpage452
dc.identifier.issn1019-5149
dc.identifier.issue3
dc.identifier.pmid38650560
dc.identifier.scopus2-s2.0-85192954253
dc.identifier.scopusqualityQ3
dc.identifier.startpage448
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.42717-22.1
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2502
dc.identifier.volume34
dc.identifier.wosWOS:001234815200005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectGlioma
dc.subjectSurgical treatment
dc.subjectHCMV
dc.subjectqPCR
dc.titleNon-Detection of HCMV Total Genomic DNA in Human Glioma Cells Genome
dc.typeArticle

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