Antioxidant, cytotoxic, anti-migratory, and pro-apoptotic effects of Bolanthus turcicus extracts on head and neck cancer cells

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dc.authoridYilmaz Sarialtin, Sezen/0000-0002-8387-4146
dc.contributor.authorOzdas, Sibel
dc.contributor.authorCanatar, Ipek
dc.contributor.authorOzdas, Talih
dc.contributor.authorSarialtin, Sezen Yilmaz
dc.contributor.authorAgca, Asli Can
dc.contributor.authorKoc, Murat
dc.date.accessioned2025-01-06T17:38:19Z
dc.date.available2025-01-06T17:38:19Z
dc.date.issued2024
dc.description.abstractPurpose Investigation of various plant extracts using in-vitro/in-vivo assays has emerged as a promising avenue for identifying potential pharmacophores that can be developed into therapeutic drugs. This study aims to assess the bioactive compounds and antioxidant capacity of the Bolanthus turcicus (B. turcicus) and to investigate the effects on head and neck cancer (HNC) cell lines. Methods Methanol (MeOH), ethyl acetate (EA) and aqueous (Aq) extracts were prepared from B. turcicus, and the amount of total phenolic content (TPC) and total flavonoid content (TFC) in the extracts were analyzed by the Folin-Ciocalteu and Aluminum chloride method, respectively. In addition, the total antioxidant capacity and iron reducing potential of B. turcicus extracts were determined by the Phosphomolybdenum and Ferric ion reducing antioxidant power (FRAP) method. The effect of B. turcicus on HEp-2, SCC-90, SCC-9, FaDu HNC cell viability, motility, and cell-nuclear morphology was evaluated by MTT, scratch-wound healing assay, and Pllalloidin-DAPI staining, respectively. The effect of B. turcicus on the expression of CASP-3, BAX, and BCL-2 genes at the mRNA, protein, and intracellular level was evaluated by quantitative PCR (qPCR), western blot, and immunofluorescence staining. Moreover, Annexin V-FITC/PI, was used in flow cytometry to investigate the effect of B. turcicus on apoptosis. Results The MeOH extract exhibited the highest phenolic content, flavonoid content and antioxidant activity (p < 0.05 for all). HNC cells treated with extracts indicated delayed wound healing and decreased motility (p < 0.05 for all). Analysis of annexin V-PI staining indicated that the B. turcicus extracts induced apoptosis but not viability and necrosis in the HNC cell (p < 0.05 for all). Moreover, qPCR data regarding the apoptotic mechanism showed that the extracts could induce apoptosis by upregulation of pro-apoptotic CASP-3 and BAX genes and downregulation of anti-apoptotic BCL-2 gene (p < 0.05 for all). The expression of protein and intracellular levels of CASP-3 and BAX were increased, while the BCL-2 was decreased in cells treated with the extracts (p < 0.05 for all). In addition, diffuse pycnosis and DNA condensation in HNC cell nuclei, confirming apoptotic cell death (p < 0.05 for all). Conclusion This study data indicated that B. turcicus extracts have antioxidant, cytotoxic, anti-migratory and pro-apoptotic activity. In conclusion, it has been shown that B. turcicus can be used as a potential therapeutic agent against HNC.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [120Z967]
dc.description.sponsorshipThis work was supported by Scientific and Technological Research Council of Turkey (TUBITAK) (Grant Number: 120Z967 | Recipient: Sibel OZDAS, PhD)
dc.identifier.doi10.1007/s11033-024-09994-5
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1
dc.identifier.pmid39476042
dc.identifier.scopus2-s2.0-85208164943
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11033-024-09994-5
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2541
dc.identifier.volume51
dc.identifier.wosWOS:001346035300004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectHead and neck cancer
dc.subjectBolanthus turcicus
dc.subjectAntioxidant
dc.subjectAnti-migratory
dc.subjectPro-apoptotic activity
dc.titleAntioxidant, cytotoxic, anti-migratory, and pro-apoptotic effects of Bolanthus turcicus extracts on head and neck cancer cells
dc.typeArticle

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