An OMIC approach to elaborate the antibacterial mechanisms of different alkaloids

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dc.authoridAVCI, Fatma Gizem/0000-0001-6618-0487
dc.contributor.authorAvci, Fatma Gizem
dc.contributor.authorSayar, Nihat Alpagu
dc.contributor.authorAkbulut, Berna Sariyar
dc.date.accessioned2025-01-06T17:36:24Z
dc.date.available2025-01-06T17:36:24Z
dc.date.issued2018
dc.description.abstractPlant-derived substances have regained interest in the fight against antibiotic resistance owing to their distinct antimicrobial mechanisms and multi-target properties. With the recent advances in instrumentation and analysis techniques, OMIC approaches are extensively used for target identification and elucidation of the mechanism of phytochemicals in drug discovery. In the current study, RNA sequencing based transcriptional profiling together with global differential protein expression analysis was used to comparatively elaborate the activities and the effects of the plant alkaloids boldine, bulbocapnine, and roemerine along with the well-known antimicrobial alkaloid berberine in Bacillus subtilis cells. The transcriptomic findings were validated by qPCR. Images from scanning electron microscope were obtained to visualize the effects on the whole-cells. The results showed that among the three selected alkaloids, only roemerine possessed antibacterial activity. Unlike berberine, which is susceptible to efflux through multidrug resistance pumps, roemerine accumulated in the cells. This in turn resulted in oxidative stress and building up of reactive oxygen species, which eventually deregulated various pathways such as iron uptake. Treatment with boldine or bulbocapnine slightly affected various metabolic pathways but has not changed the growth patterns at all. (C) 2018 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipMarmara University, Scientific Research Projects Committee [FEN-A-050614-0238]; Scientific and Technological Research Council of Turkey [TUBITAK-113M052]
dc.description.sponsorshipThis work was supported by Marmara University, Scientific Research Projects Committee (FEN-A-050614-0238) and The Scientific and Technological Research Council of Turkey (TUBITAK-113M052). We would like to express our special thanks to Kubra Karaosmanoglu Yoneten, Ceyhun Bereketoglu, and Kazim Yalcin Arga for their kind help.
dc.identifier.doi10.1016/j.phytochem.2017.12.023
dc.identifier.endpage131
dc.identifier.issn0031-9422
dc.identifier.pmid29494814
dc.identifier.scopus2-s2.0-85042510144
dc.identifier.scopusqualityQ1
dc.identifier.startpage123
dc.identifier.urihttps://doi.org/10.1016/j.phytochem.2017.12.023
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1862
dc.identifier.volume149
dc.identifier.wosWOS:000430778200011
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofPhytochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectAporphine alkaloid
dc.subjectRoemerine
dc.subjectAntibacterial
dc.subjectRNA-Seq
dc.subjectProteomics
dc.subjectBacillus subtilis
dc.titleAn OMIC approach to elaborate the antibacterial mechanisms of different alkaloids
dc.typeArticle

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