The Effect of Ascorbic Acid over the Etoposide- and Temozolomide-Mediated Cytotoxicity in Glioblastoma Cell Culture: A Molecular Study

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dc.authoridKelebek, Hasim/0000-0002-8419-3019
dc.authoridceylan, seda/0000-0002-1088-7886
dc.authoridGokturk, Dilek/0000-0002-1195-5828
dc.contributor.authorGokturk, Dilek
dc.contributor.authorKelebek, Haşim
dc.contributor.authorCeylan, Seda
dc.contributor.authorYilmaz, Dervis Mansuri
dc.date.accessioned2025-01-06T17:38:09Z
dc.date.available2025-01-06T17:38:09Z
dc.date.issued2018
dc.description.abstractAIM: Glioblastoma (GBM) is one of the lethal central nervous system tumors. One of the widely used chemical agents for the treatment of glioblastoma is temozolomide. It is an orally administered, deoxyribonucleic acid (DNA) alkylating agent. DNA alkylation triggers the death of tumor cells. However, some tumor cells are able to repair this type of DNA damage and thus lower the therapeutic effect of temozolomide. Laboratory and clinical studies indicate that temozolomide's anticancer effects might be strengthened when combined with other chemotherapeutic agents like etoposide or antioxidant agents like ascorbic acid. In this study, we aimed to evaluate the cytotoxic and oxidative stress effects of ascorbic acid (1000 mu M), temozolomide (100 mu M) and etoposide (25 mu M) agents alone and in dual and triple combinations in a glioblastoma U87 MG cell culture. MATERIAL and METHODS: The cytotoxic and oxidative stress effects were investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis methods. RESULTS: Cytotoxicity tests showed that etoposide, temozolomide, etoposide+ascorbic acid, temozolomide+ascorbic acid, temozolomide+etoposide and temozolomide+etoposide+ascorbic acid combinations have anti-proliferative effects. The maximum anti-proliferation response was observed in the temozolomide+etoposide+ascorbic acid-added group. Similarly LCMS/MS analyses showed that minimum oxidative DNA damage occurred in the temozolomide+etoposide+ascorbic acid-added group. CONCLUSION: Ascorbic acid decreases the cytotoxic and genotoxic effect of etoposide and etoposide-temozolomide combination but it has no meaningful effect on temozolomide's toxicity.
dc.description.sponsorshipScientific Research Projects Fund of Adana Science and Technology University [MUHDBF.BIYO.2014-5]
dc.description.sponsorshipThis work was supported by Scientific Research Projects Fund of Adana Science and Technology University (Project no: MUHDBF.BIYO.2014-5). The authors thank Assist. Prof. Tugba Bagci Onder from Koc University, Faculty of Medicine, Molecular Biology Department for kindly donating the U 87 MG Glioblastoma cell line.
dc.identifier.doi10.5137/1019-5149.JTN.19111-16.1
dc.identifier.endpage18
dc.identifier.issn1019-5149
dc.identifier.issue1
dc.identifier.pmid28191621
dc.identifier.scopus2-s2.0-85046637490
dc.identifier.scopusqualityQ3
dc.identifier.startpage13
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.19111-16.1
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2501
dc.identifier.volume28
dc.identifier.wosWOS:000423394400003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectGlioblastoma
dc.subjectAscorbic acid
dc.subjectCytotoxicity
dc.subjectEtoposide
dc.subjectTemozolomide
dc.titleThe Effect of Ascorbic Acid over the Etoposide- and Temozolomide-Mediated Cytotoxicity in Glioblastoma Cell Culture: A Molecular Study
dc.typeArticle

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