Nuclear entrapment of p33ING1b by inhibition of exportin-1: A trigger of apoptosis in head and neck squamous cell cancer

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dc.authoridOZDAS, Sibel/0000-0003-4610-2785
dc.contributor.authorOzdas, Sibel
dc.date.accessioned2025-01-06T17:38:20Z
dc.date.available2025-01-06T17:38:20Z
dc.date.issued2018
dc.description6th International Congress of Molecular Medicine -- MAY 22-25, 2017 -- Istanbul, TURKEY
dc.description.abstractThe effect of deregulation of nuclear export mediated by exportin-L with consequent cellular mislocalization of p33(ING1b), a member of the tumor suppressor gene family, has not been previously investigated in head and neck squamous cell cancer (IINSCC). We evaluated the effect of reversing cytoplasmic p33(ING1b) localization through inhibition of exportin-1 by leptomycin B (LMB) and the effect of nuclear entrapment of p33(ING1b) on molecular alterations in primary and metastatic IINSCC lines. The expression and location of exportin-1 and p33(ING1b) We re analyzed by a quantitative real-time reverse transcription polymerase chain reaction PCR (qRT-PCR), a Western blot, and iminunostaining. Cell proliferation and migration assays were conducted to determine the effect of exportin-1 inhibition on the cell lines. Exportin-1 was overexpressed in metastatic FINSCC, whereas p33(ING1) was poorly expressed. Exportin-1 inhibition induced nuclear entrapment and upregulation of p33(ING1b), extensive apoptosis, and growth arrest. It also suppressed cell migration. Cytoplasmic p33(ING1b)-mediated regulation of cell growth and nuclear entrapment of p33(ING1b) via inhibition of exportin-1 may be a key mechanism for inducing IINSCC apoptosis.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [215S087]; Adana Science and Technology University Scientific Research Projects Coordination Unit [16103018]
dc.description.sponsorshipThe author expresses my gratitude to Talih Ozdas, MD from the Adana City Hospital, Otolaryngology Clinic, Adana. Turkey, for his help in the preparation of the manuscript. This study was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) (Project No. 215S087) and the Adana Science and Technology University Scientific Research Projects Coordination Unit (Project No: 16103018). This study was presented at VI. International Congress of Molecular Medicine, Istanbul, Turkey, 22-25 May, 2017.
dc.identifier.doi10.14715/cmb/2018.64.5.11
dc.identifier.endpage72
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue5
dc.identifier.pmid29729696
dc.identifier.scopus2-s2.0-85046688317
dc.identifier.scopusqualityQ4
dc.identifier.startpage66
dc.identifier.urihttps://doi.org/10.14715/cmb/2018.64.5.11
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2543
dc.identifier.volume64
dc.identifier.wosWOS:000437411400011
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherC M B Assoc
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjecthead and neck cancer
dc.subjectExportin-1
dc.subjectp33(ING1b)
dc.subjectNuclear export
dc.subjectLeptomycin B
dc.titleNuclear entrapment of p33ING1b by inhibition of exportin-1: A trigger of apoptosis in head and neck squamous cell cancer
dc.typeConference Object

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