Assessment of Anticancer Effects of Aloe vera on 3D Liver Tumor Spheroids in a Microfluidic Platform

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dc.authoridTevlek, Atakan/0000-0003-0601-8642
dc.contributor.authorTevlek, Atakan
dc.contributor.authorKibar, Gunes
dc.contributor.authorCetin, Barbaros
dc.date.accessioned2026-02-27T07:33:17Z
dc.date.available2026-02-27T07:33:17Z
dc.date.issued2025
dc.description.abstractThe search for effective anticancer therapies has increasingly focused on natural compounds like Aloe vera, renowned for its therapeutic properties. This study investigates the anticancer properties of Aloe vera on 3D liver tumor spheroids via a PDMS-based microfluidic device, providing a more physiologically realistic model compared to traditional 2D cultures. HepG2 cells were cultivated to generate 3D spheroids on-chip, thereafter subjected to different concentrations of Aloe vera and the chemotherapeutic drug Doxorubicin to evaluate cytotoxic effects. The microfluidic system, validated by COMSOL simulations, facilitated continuous perfusion and real-time assessment of cell viability over a duration of 10 days. The results indicated that Aloe vera markedly diminished cell viability by triggering apoptosis at concentrations over 12.5 mg/mL. IC50 values were determined at 72 h: 25 +/- 0.10 mg/mL for Aloe vera and 5.47 +/- 0.03 mu g/mL for Doxorubicin in 2D cultures, but in 3D cultures, the IC50 values were 31.25 +/- 0.14 mg/mL for Aloe vera and 8.33 +/- 0.05 mu g/mL for Doxorubicin. This study underscores the promise of Aloe vera as a natural anticancer agent and illustrates the efficacy of microfluidic platforms for enhanced drug screening and customized medicine applications.
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu
dc.description.sponsorshipThe study was supported by the Turkiye Bilimsel ve Teknolojik Arastirma Kurumu.
dc.identifier.doi10.1002/bit.29033
dc.identifier.endpage2608
dc.identifier.issn0006-3592
dc.identifier.issn1097-0290
dc.identifier.issue9
dc.identifier.pmid40539369
dc.identifier.startpage2592
dc.identifier.urihttp://dx.doi.org/10.1002/bit.29033
dc.identifier.urihttps://hdl.handle.net/20.500.14669/4529
dc.identifier.volume122
dc.identifier.wosWOS:001511953500001
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology and Bioengineering
dc.relation.publicationcategoryMakale - Uluslararas� Hakemli Dergi - Kurum ��retim Eleman�
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20260302
dc.subject3D cell culture
dc.subjectAloe vera
dc.subjectanticancer agent
dc.subjectmicrofluidic
dc.titleAssessment of Anticancer Effects of Aloe vera on 3D Liver Tumor Spheroids in a Microfluidic Platform
dc.typeArticle

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