Green synthesis of silver nanoparticles using carob leaf extract: Characterization and analysis of toxic effects in model organism Galleria mellonella L. (The greater wax moth)

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dc.contributor.authorAndırın, Aslıhan
dc.contributor.authorYaycı, Nur Dudu
dc.contributor.authorIdikut, Murat
dc.contributor.authorKara, Ayse
dc.contributor.authorTuncsoy, Mustafa
dc.contributor.authorTuncsoy, Benay
dc.contributor.authorOzalp, Pınar
dc.date.accessioned2025-01-06T17:30:00Z
dc.date.available2025-01-06T17:30:00Z
dc.date.issued2024
dc.description.abstractSilver nanoparticles (Ag NPs) have been used in many studies due to their inhibitory properties on microorganisms such as bacteria and viruses. In recent years, due to global problems such as environmental pollution, the green synthesis (biosynthesis) method is frequently preferred because it is simple and low cost and does not require the use of toxic substances. The aim of this study is to synthesize silver nanoparticles (Ag NPs) from Ceratonia siliqua L. leaves and investigate their antioxidant and immunotoxic properties using Galleria mellonella last instar larvae. The UV spectrophotometer, TEM, XRD and FTIR measurements were used to characterize the Ag NPs. In this study, it was determined that the effects on antioxidant enzyme activities (SOD, CAT, GPx, GST), acetylcholinesterase (AChE) and total hemocyte count (THC) as well as phenoloxidase activity determine their effect on antioxidant defence and the immune system in model organism G. mellonella larvae. We observed that green synthesized Ag NPs accumulate in the midgut of the larvae and led to the increasing of CAT and SOD activities. GST and AChE activities were increased in the fat body of the larvae; otherwise, it was decreased in the midgut. Moreover, increases were found in THC and phenoloxidase activity. Consequently, green synthesized silver nanoparticles led to oxidative stress and immunotoxic effects on G. mellonella larvae. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
dc.identifier.doi10.1007/s11356-024-34996-y
dc.identifier.endpage57788
dc.identifier.issn0944-1344
dc.identifier.issue47
dc.identifier.pmid39294535
dc.identifier.scopus2-s2.0-85204227512
dc.identifier.scopusqualityQ1
dc.identifier.startpage57778
dc.identifier.urihttps://doi.org/10.1007/s11356-024-34996-y
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1446
dc.identifier.volume31
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofEnvironmental Science and Pollution Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectAntioxidant activity
dc.subjectCarob
dc.subjectGreen synthesis
dc.subjectImmunotoxicity
dc.subjectSilver nanoparticles
dc.titleGreen synthesis of silver nanoparticles using carob leaf extract: Characterization and analysis of toxic effects in model organism Galleria mellonella L. (The greater wax moth)
dc.typeArticle

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