Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes

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dc.contributor.authorDerici, Gizem Ece
dc.contributor.authorÖzdaş, Sibel
dc.contributor.authorCanatar, Ipek
dc.contributor.authorKoç, Murat
dc.date.accessioned2025-01-06T17:30:13Z
dc.date.available2025-01-06T17:30:13Z
dc.date.issued2021
dc.description.abstractDiabetes mellitus (DM) is a metabolic disorder with chronic hyperglycemia featured by metabolic outcomes owing to insufficient insulin secretion and/or insulin effect defect. It is critical to investigate new therapeutic approaches for T2DM and alternative, natural agents that target molecules in potential signal pathways. Medicinal plants are significant resources in the research of alternative new drug active ingredients. Bolanthus spergulifolius (B. spergulifolius) is one of the genera of the family Caryophyllaceae. In this study, it was explored the potential anti-diabetic effects in vitro of B. spergulifolius extracts on 3T3-L1 adipocytes. The total phenolic contents (TPC) of methanolic (MeOH), ethyl acettate (EA) and aqueous extracts of B. spergulifolius were evaluated via Folin-Ciocateau. B. spergulifolius extracts showing highly TPC (Aqueous< MeOH< EA) and their different concentrations were carried out on preadipocytes differentiated in to mature 3T3-L1 adipocytes to investigate their halfmaximal (50%) inhibitory concentration (IC50) value by using Thiazolyl blue tetrazolium bromide (MTT) assay. The IC50 of MeOH, EA and Aqueous extracts were observed as 305.7 ± 5.583 ?g/mL, 567.4 ± 3.008 ?g/mL, and 418.3 ± 4.390 ?g/mL and used for further experiments. A live/dead assay further confirmed the cytotoxic effects of MeOH, EA and Aqueous extracts (respectively, 69.75 ± 1.70%, 61.75 ± 1.70%, 70 ± 4.24%, and for all p< 0.05). Also, effects of extracts on lipid accumulation in mature 3T3-L1 adipocytes were evaluated by Oil- Red O staining assay. The extracts effectively decreased lipid-accumulation compared to untreated adipocytes (for all p< 0.05). Moreover, effect of extracts on apoptosis regulated by the Bax and Bcl-2 was investigated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The extracts significantly induced apoptosis by up-regulating pro-apoptotic Bax expression but down-regulated anti-apoptotic Bcl-2 gene expression compared to untreated adipocytes (for all p< 0.05). The Glut-4 expression linked with insulin resistance was determined by qRT-PCR, Western-blot analysis, and immunofluorescence staining. In parallel, the expression of Glut-4 in adipocytes treated with extracts was significantly higher compared to untreated adipocytes (for all p< 0.05). Extracts significantly suppressed cell migration after 30 h of wounding in a scratch-assay (for all p< 0.05). Cell morphology and diameter were further evaluated by phase-contrast microscopy, scanning electron microscopy, Immunofluorescence with F-Actin and Giemsa staining. The adipocytes treated with extracts partially lost spherical morphology and showed smaller cell-diameter compared to untreated adipocytes (for all p< 0.05). In conclusion, these results suggest that extracts of B. spergulifolius cause to an induce apoptosis, decrease lipid-accumulation, wound healing, up-regulating Glut-4 level and might contribute to reducing of insulin-resistance in DM. © 2021 Derici et al.
dc.identifier.doi10.1371/journal.pone.0252707
dc.identifier.issn1932-6203
dc.identifier.issue6 June 2021
dc.identifier.pmid34133443
dc.identifier.scopus2-s2.0-85108298219
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0252707
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1529
dc.identifier.volume16
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.ispartofPLoS ONE
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subject3T3-L1 Cells
dc.subjectAdipocytes
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectCaryophyllaceae
dc.subjectCell Survival
dc.subjectGene Expression
dc.subjectGlucose Transporter Type 4
dc.subjectHypoglycemic Agents
dc.subjectInsulin Resistance
dc.subjectMice
dc.subjectMicroscopy, Fluorescence
dc.subjectPlant Extracts
dc.subjectPlants, Medicinal
dc.subjectProto-Oncogene Proteins c-bcl-2
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectantidiabetic agent
dc.subjectglucose transporter 4
dc.subjectplant extract
dc.subjectprotein bcl 2
dc.subject3T3-L1 cell line
dc.subjectadipocyte
dc.subjectanimal
dc.subjectCaryophyllaceae
dc.subjectcell survival
dc.subjectchemistry
dc.subjectcytology
dc.subjectdrug effect
dc.subjectfluorescence microscopy
dc.subjectgene expression
dc.subjectgenetics
dc.subjectinsulin resistance
dc.subjectisolation and purification
dc.subjectmedicinal plant
dc.subjectmetabolism
dc.subjectmouse
dc.subjectreverse transcription polymerase chain reaction
dc.subjectWestern blotting
dc.titleAntidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes
dc.typeArticle

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