The use of CRISPR-Cas-based systems in bacterial cell factories

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dc.authoridAKKALE, CENGIZ/0000-0002-9537-7104
dc.authoridKaya, Hilal Betul/0000-0002-2543-7212
dc.contributor.authorKolasinliler, Gulsen
dc.contributor.authorAagre, Mehdi Movahed
dc.contributor.authorAkkale, Cengiz
dc.contributor.authorKaya, Hilal Betul
dc.date.accessioned2025-01-06T17:37:16Z
dc.date.available2025-01-06T17:37:16Z
dc.date.issued2023
dc.description.abstractClustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas system has been the focus since its first introduction to genome editing research. It acts as an adaptive immune system in many prokaryotes by detecting and degrading viral/invasive DNA/RNA repurposed for targeted genome editing in various organisms, including bacterial species relevant to industrial biotechnology. Targeted genome editing in bacteria has been implemented for multiple purposes, such as method development, understanding knowledge of metabolic processes and improving the productivity of industrial products. With the increasing share of biotechnological products, bac-teria have been the subject in the field of genome editing.This review summarizes the immunological mechanisms of CRISPR-Cas systems, the variation in classification of CRISPR-Cas systems by highlighting their features and the current applications of CRISPR-based genome editing in industrially relevant bacteria. In addition, an extensive literature review focused on recent advances has been presented in bacterial species according to industrial significance.
dc.description.sponsorshipManisa Celal Bayar University Scien- tific Research Projects [2020-123]
dc.description.sponsorshipFunding This study was funded by the Manisa Celal Bayar University Scien- tific Research Projects (2020-123) .
dc.identifier.doi10.1016/j.bej.2023.108880
dc.identifier.issn1369-703X
dc.identifier.issn1873-295X
dc.identifier.scopus2-s2.0-85149779975
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.bej.2023.108880
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2174
dc.identifier.volume194
dc.identifier.wosWOS:000961757500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectCRISPR
dc.subjectGenome editing
dc.subjectCas protein
dc.subjectIndustrial production
dc.titleThe use of CRISPR-Cas-based systems in bacterial cell factories
dc.typeArticle

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