Monolithic hydrophobic cryogel columns for protein separation

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dc.authoridODABASI, Mehmet/0000-0002-3288-132X
dc.authoridBaydemir Pesint, Gozde/0000-0001-8668-8296
dc.authoridZenger, Okan/0000-0002-5669-0325
dc.contributor.authorErzengin, Mahmut
dc.contributor.authorBaydemir Pesint, Gozde
dc.contributor.authorZenger, Okan
dc.contributor.authorOdabasi, Mehmet
dc.date.accessioned2025-01-06T17:43:17Z
dc.date.available2025-01-06T17:43:17Z
dc.date.issued2022
dc.description.abstractThe present study was conducted for the synthesis of a novel supermacroporous monolithic hydrophobic adsorbent for lysozyme (Lyz) selected as a model protein from aqueous solution. After preparation of poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(l)-tyrosine methyl ester) monolithic cryogel column, 1-naphthylamine was covalently attached, and the prepared column was abbreviated as NA-Mcc. Scanning electron microscopy, Fourier transform infrared spectroscopy and Brunauer, Emmett and Teller device were utilized for the morphology, functional groups and surface area measurements of the column. Effects of several parameters including Lyz content of the adsorption solution, pH of the medium used, ambient temperature, type of salt and flow rate on the adsorption capacity of the polymeric material were examined in continuous operation. The maximum value achieved for Lyz adsorption from aqueous phase was found to be 105.8 mg/g in phosphate buffer. In addition, NA-Mcc was investigated in terms of reusability, and it was demonstrated that there is no significant change in the adsorption properties of prepared monolithic hydrophobic cryogels after 30 adsorption-desorption cycles.
dc.identifier.doi10.1007/s00289-021-03568-2
dc.identifier.endpage1499
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85100559094
dc.identifier.scopusqualityQ1
dc.identifier.startpage1485
dc.identifier.urihttps://doi.org/10.1007/s00289-021-03568-2
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2591
dc.identifier.volume79
dc.identifier.wosWOS:000615772900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectHydrophobic interaction chromatography
dc.subjectEnzyme purification
dc.subjectLysozyme
dc.subjectMonolithic cryogel column
dc.titleMonolithic hydrophobic cryogel columns for protein separation
dc.typeArticle

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