Extraction of pectin from albedo of lemon peels for preparation of tissue engineering scaffolds

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dc.authoridceylan, seda/0000-0002-1088-7886
dc.authoriddemir, didem/0000-0002-2977-2077
dc.authoridGokturk, Dilek/0000-0002-1195-5828
dc.authoridBolgen, Nimet/0000-0003-3162-0803
dc.contributor.authorDemir, Didem
dc.contributor.authorCeylan, Seda
dc.contributor.authorGokturk, Dilek
dc.contributor.authorBolgen, Nimet
dc.date.accessioned2025-01-06T17:37:48Z
dc.date.available2025-01-06T17:37:48Z
dc.date.issued2021
dc.description.abstractPectin is a type of anionic polysaccharide naturally found in a number of fruits and vegetables. Although pectin is widely used for food industry, its biomedical applications such as wound dressing, drug delivery and cancer targeting have also been investigated. In our study, we combined extracted pectin (from albedo of lemon peels) with chitosan (as a natural polymer) to synthesize chitosan/pectin cryogels. The extracted pectin was subjected to qualitative and quantitative analyses. Chitosan/pectin spongy supermacroporous cryogels were produced by cryogelation method at different combinations (100:0, 80:20, 60:40 and 40:60, w/w). Polyelectrolyte interactions between pectin and chitosan and crosslinking of chitosan with glutaraldehyde were verified by using FTIR. The porosity, swelling ratio, degradation behaviors and mechanical properties of cryogels were determined. SEM analysis demonstrated the pore morphology and average pore diameters of cryogels. After all analysis, 40:60 chitosan/pectin cryogel was selected for cytotoxicity studies. Glioblastoma (U-87 MG) cell line was used to evaluate the in vitro cytotoxicity of scaffolds. MTT assay and SEM analyses demonstrated the scaffolds were nontoxic, and supported cell attachment and viability.
dc.identifier.doi10.1007/s00289-020-03208-1
dc.identifier.endpage2226
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85084129380
dc.identifier.scopusqualityQ1
dc.identifier.startpage2211
dc.identifier.urihttps://doi.org/10.1007/s00289-020-03208-1
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2365
dc.identifier.volume78
dc.identifier.wosWOS:000529119300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectExtraction
dc.subjectLemon
dc.subjectPectin
dc.subjectChitosan
dc.subjectCryogel
dc.subjectCytotoxicity
dc.titleExtraction of pectin from albedo of lemon peels for preparation of tissue engineering scaffolds
dc.typeArticle

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