Recombinant D-tagatose 3-epimerase production and converting fructose into allulose

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dc.contributor.authorParildi, Erva
dc.contributor.authorKola, Osman
dc.contributor.authorOzcan, Bahri Devrim
dc.contributor.authorAkkaya, Murat Reis
dc.contributor.authorDikkaya, Elif
dc.date.accessioned2025-01-06T17:44:55Z
dc.date.available2025-01-06T17:44:55Z
dc.date.issued2022
dc.description.abstractIn this study, D-tagatose 3-epimerase gene was amplified from Escherichia coli JM109 by PCR and re-cloned into E. coli JM109 using HincII digested pUC18 cloning vector. Digestion of recombinant plasmid by HincII and PCR amplification of the gene from recombinant plasmid produced 789 bp gene band on agarose gel which indicated the gene integration. The host cell was grown in a shaking incubator at 37 degrees C for 24 hr and then the optical density (OD) of the cells was measured in a spectrophotometer at 600 nm wavelengths. When the OD600 value reached 0.61, the protein expression was induced by the addition of 0.1 mM IPTG into the growth medium. Through His-select gel column purification, highly purified and stable DTE protein was produced. The conversion rate of D-fructose into D-allulose was determined by HPLC. The native and recombinant enzymes could effectively convert D-fructose into D-allulose with a turnover ratio of 20.76%. Novelty impact statement A recombinant enzyme, D-tagatose 3-epimerase, was produced and D-fructose was converted into D-allulose using this recombinant enzyme.
dc.description.sponsorshipREPUBLIC OF TURKEY MINISTRY OF AGRICULTURE AND FORESTRY GENERAL DIRECTORATE OF AGRICULTURAL RESEARCH AND POLICIES [TAGEM-18/AR-GE/30]
dc.description.sponsorshipREPUBLIC OF TURKEY MINISTRY OF AGRICULTURE AND FORESTRY GENERAL DIRECTORATE OF AGRICULTURAL RESEARCH AND POLICIES, Grant/Award Number: TAGEM-18/AR-GE/30
dc.identifier.doi10.1111/jfpp.15508
dc.identifier.issn0145-8892
dc.identifier.issn1745-4549
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85104527011
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1111/jfpp.15508
dc.identifier.urihttps://hdl.handle.net/20.500.14669/3246
dc.identifier.volume46
dc.identifier.wosWOS:000641684300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Food Processing and Preservation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectD-Psıcose 3-Epımerase
dc.subjectSugar D-Allulose
dc.subjectPurıfıcatıon
dc.subjectBıoconversıon
dc.subjectExpressıon
dc.subjectObesıty
dc.subjectClonıng
dc.titleRecombinant D-tagatose 3-epimerase production and converting fructose into allulose
dc.typeArticle

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