Molecularly imprinted composite discs for transferrin recognition

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dc.authoridBaydemir Pesint, Gozde/0000-0001-8668-8296
dc.authoridZenger, Okan/0000-0002-5669-0325
dc.contributor.authorAylaz, Gulgun
dc.contributor.authorZenger, Okan
dc.contributor.authorBaydemir Pesint, Gozde
dc.contributor.authorAndac, Muge
dc.date.accessioned2025-01-06T17:43:38Z
dc.date.available2025-01-06T17:43:38Z
dc.date.issued2022
dc.description.abstractHuman Transferrin (HTr) imprinted composite cryogel (HTr-MIPCC) discs were synthesized with distinctive structure and increased adsorption capacity and specificity for HTr by combining the advantages of the surface imprinting technique. HEMA-based cryogel was embedded with HTr-surface imprinted particles. Thanks to the imprinted particles embedding, both the higher surface area and specific recognition are provided. The structure of the HTr-MIPCC was characterized by FTIR Spectrometer, SEM, swelling studies, flow dynamics, and surface area measurements. The addition of surface imprinted particles into the cryogel disc has resulted in an increased surface area, enhancing the capacity of composite cryogel for HTr adsorption up to 10.96 mg/g and gel fractionation yields reached up to 83%. Selectivity studies were carried out against HIgG and HSA as competitors. The HTr-MIPCC discs were packaged into an FPLC column for selective depletion of HTr. It was calculated that the HTr-MIPCC is 1.071 and 1.062 times selective for HTr than HSA and HIgG, respectively. According to FPLC studies HTr-MIPCC can be considered as an excellent alternative for affinity matrixes used for the detection and selective recognition of HTr directly from human serum. HTr-MIPCC discs can be used several times without any noteworthy reduction in the adsorption capacity of HTr.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114Z664]
dc.description.sponsorshipThis project was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) within the scope of 3001-Initial R&D Projects Support Program [project number 114Z664].
dc.identifier.doi10.1080/01496395.2021.1990950
dc.identifier.endpage1375
dc.identifier.issn0149-6395
dc.identifier.issn1520-5754
dc.identifier.issue9
dc.identifier.scopus2-s2.0-85117291150
dc.identifier.scopusqualityQ2
dc.identifier.startpage1359
dc.identifier.urihttps://doi.org/10.1080/01496395.2021.1990950
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2751
dc.identifier.volume57
dc.identifier.wosWOS:000708714500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofSeparation Science and Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectMolecular imprinting
dc.subjectcomposite discs
dc.subjectchromatography application
dc.subjecttransferrin
dc.subjectprotein recognition
dc.titleMolecularly imprinted composite discs for transferrin recognition
dc.typeArticle

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