Microfluidic rapid isolation and electrochemical detection of S. pneumonia via aptamer-decorated surfaces

Basit Öğe Kaydı
dc.authoridKIBAR, GUNES/0000-0002-2586-6770
dc.contributor.authorBabaie, Zahra
dc.contributor.authorKibar, Gunes
dc.contributor.authorYesilkaya, Hasan
dc.contributor.authorAmrani, Yassine
dc.contributor.authorDogan, Soner
dc.contributor.authorTuna, Bilge G.
dc.contributor.authorOzalp, Veli C.
dc.contributor.authorCetin, Barbaros
dc.date.accessioned2025-04-09T12:32:06Z
dc.date.available2025-04-09T12:32:06Z
dc.date.issued2025
dc.description.abstractBackground: S. pneumoniae is widely recognized as a leading cause of respiratory infections worldwide, often resulting in high mortality rates. However, the advent of microfluidic technologies has brought significant advancements, including the simplified, sensitive, cost-effective, and rapid approach to pneumococcal bacteremia detection. In this study, a microfluidic magnetic platform is presented for rapid isolation, and an electrode array is utilized for the electrochemical detection of S. pneumoniae. Aptamer-decorated surfaces were employed for both isolation and detection. For isolation, silica magnetic microparticles were synthesized and decorated with aptamer. Results: Isolation performance was assessed for phosphate-buffered saline (PBS) and blood samples for different concentrations of S. pneumoniae. Electrical impedance spectroscopy (EIS) with fabricated gold interdigitated electrodes (IDEs) decorated with aptamer was implemented for the detection of S. pneumoniae at different bacteria concentrations. The microfluidic platform performed bacteria isolation at comparable isolation efficiency with batch systems but at a much faster rate (isolation took about a minute, and the aptamer-decorated electrode array exhibited a limit of detection (LOD) at 962 CFU/mL and linear range between 104 and 107CFU/mL. Significance: Our method represents a significant advancement compared to previous reports. Our microfluidic platform can efficiently isolate 60 mu L of the bacteria sample within about one minute. The entire process takes about two minutes including the detection step. Furthermore, our method achieves a notable improvement in the detection limit for S. pneumoniae compared to conventional ELISA and magnetic microfluidics ELISA.
dc.identifier.doi10.1016/j.aca.2025.343726
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.trdizinid40015771
dc.identifier.urihttp://dx.doi.org/10.1016/j.aca.2025.343726
dc.identifier.urihttps://hdl.handle.net/20.500.14669/4314
dc.identifier.volume1345
dc.identifier.wosWOS:001423352500001
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20250385
dc.subjectMicrofluidics
dc.subjectBiosensor
dc.subjectBacteria isolation
dc.subjectBacteria detection
dc.subjectAptamer
dc.titleMicrofluidic rapid isolation and electrochemical detection of S. pneumonia via aptamer-decorated surfaces
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu