Biochemical properties of ?-glucosidase from Turkish Hacihaliloglu apricot (Prunus armenica L.) as affected by harvest year

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dc.contributor.authorUnal, Mustafa Umit
dc.contributor.authorSener, Aysun
dc.date.accessioned2025-01-06T17:38:19Z
dc.date.available2025-01-06T17:38:19Z
dc.date.issued2017
dc.description.abstractbeta-glucosidase was extracted and purified from Turkish Hacihaliloglu apricot variety and some of its biochemical properties were studied in a two-year study (2008 and 2009). Two isoenzymes (isoenzyme A and B) were obtained upon ammonium sulfate fractionation, ion exchange chromatography using DEAE-Toyopearl 650 M and gel filtration chromatography using Sephadex G-100. The pH optimum was found to be 5.0 for both isoenzymes. The optimum temperature for isoenzyme A activity in both years was found to be 50 degrees C, whereas those for the isoenzyme B in 2008 and 2009 were 50 degrees C and 55 degrees C, respectively. Km values of the isoenzyme A and B were found to be 2.49 and 1.12 mM in 2008, and 2.41 and 1.06 mM in 2009, respectively. The Z values for isoenzyme A in 2008 and 2009 were found to be 20.3 degrees C and 17.9 degrees C, respectively. Those for isoenzyme B in 2008 and 2009 were 21.1 degrees C and 23.0 degrees C, respectively. Ea values for isoenzyme A were 108.1 kjimolK and 1223 kj/molK, while those for isoenzyme B were found to be 103.5 kj/mol K and 95.1 kjimolK in 2008 and 2009, respectively. Of the inhibitors tested, CaCl2 was the most effective. (C) 2017 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipResearch Fund of the University of Cukurova, Turkey [ZF2008D4]
dc.description.sponsorshipThis study was funded by the Research Fund of the University of Cukurova (ZF2008D4), Turkey.
dc.identifier.doi10.1016/j.lwt.2017.01.033
dc.identifier.endpage196
dc.identifier.issn0023-6438
dc.identifier.issn1096-1127
dc.identifier.scopus2-s2.0-85009895186
dc.identifier.scopusqualityQ1
dc.identifier.startpage190
dc.identifier.urihttps://doi.org/10.1016/j.lwt.2017.01.033
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2533
dc.identifier.volume79
dc.identifier.wosWOS:000395603100023
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofLwt-Food Science and Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectHacihaliloglu apricot
dc.subjectbeta-glucosidase
dc.subjectHarvest year
dc.subjectKinetics
dc.subjectThermal inactivation
dc.titleBiochemical properties of ?-glucosidase from Turkish Hacihaliloglu apricot (Prunus armenica L.) as affected by harvest year
dc.typeArticle

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