An in vitro evaluation of Genipin-crosslinked and Hypericum perforatum incorporated novel membranes for skin tissue engineering applications

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dc.authoridceylan, seda/0000-0002-1088-7886
dc.contributor.authorCeylan, Seda
dc.date.accessioned2025-01-06T17:36:58Z
dc.date.available2025-01-06T17:36:58Z
dc.date.issued2021
dc.description.abstractIncorporating medicinal plant extracts in membranes have a great potential as scaffolds for tissue engineering applications or vehicles for delivering therapeutic agents. Herein, Hypericum perforatum oil (0.25, 0.50, % vol/vol) loaded membranes were developed with Polyvinyl alcohol and chitosan polymer, where Genipin works as a chemical crosslinker to obtain a wound dressing material with acceptable characterization properties. Chemical groups, surface morphology, water uptake capacity, water vapor permeability rate, hydrophilicity, and mechanical properties of membranes were thoroughly investigated. Increasing oil concentration had a significant effect on the water uptake, surface morphology. and water vapor permeability rate of the membranes. Cytocompatibility of the membrane was also investigated with mouse embryonic fibroblasts (MEF) by 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for direct and indirect cell culture studies. SEM was used to investigate the cell morphology on the membranes. The MTT assay findings prove that Genipin crosslinked H. perforatum oil loaded scaffolds are highly biocompatible and enhance the adhesion and proliferation of MEF cells. In addition to this, the genotoxicity test was performed to show DNA fragmentation. Results showed that the H. perforatum oil loaded polyvinyl alcohol-chitosan membrane presents suitable properties for potential skin tissue engineering applications.
dc.description.sponsorshipScientific Research Projects Unit of Adana Alparslan Turke Science and Technology University [BAP-20103004]
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Unit of Adana Alparslan Turke Science and Technology University (BAP-20103004). SEM images were obtained from CUMER laboratories of Cukurova University, contact angle measurements were measured in BIOMATEN laboratories of Orta Dou Teknik universitesi. The author also would like to express their special gratitude to research assistant M. Damla ozdemir for her invaluable advices about cytocompatibility studies and supporting in experimental procedure of cell investigations.
dc.identifier.doi10.1002/app.51385
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue43
dc.identifier.scopus2-s2.0-85109375933
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/app.51385
dc.identifier.urihttps://hdl.handle.net/20.500.14669/2055
dc.identifier.volume138
dc.identifier.wosWOS:000672117200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectChitosan
dc.subjectHypericum perforatum
dc.subjectPVA
dc.titleAn in vitro evaluation of Genipin-crosslinked and Hypericum perforatum incorporated novel membranes for skin tissue engineering applications
dc.typeArticle

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