Evaluation of PVA/Chitosan cryogels as potential tissue engineering scaffolds; synthesis, cytotoxicity, and genotoxicity investigations

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dc.contributor.authorCeylan, Seda
dc.contributor.authorAlatepeli, Burcu
dc.date.accessioned2025-01-06T17:30:15Z
dc.date.available2025-01-06T17:30:15Z
dc.date.issued2021
dc.description.abstractCryogelation has become an advantageous method to obtain macro-porous materials with well-defined, interconnected pores for tissue engineering applications. Herein, polyvinyl alcohol and chitosan polymers (PVA-CHI) were used to produce cryogel scaffolds via cryogelation. Glutaraldehyde was used as a crosslinking agent and the effect of crosslinking amount on the properties of scaffolds investigated. Glutaraldehyde amount was divided into 5, 10, and 15% total amount of polymer concentration. The optimized pore morphology was obtained as a scaffold containing 5% glutaraldehyde amount. In addition to the FTIR, SEM, swelling, and degradation analyses, mechanical tests were performed to present the characterization properties of the cryogels. Direct and indirect cytotoxicity test and genotoxicity experiments were performed with Mouse Embryonic Fibroblasts (MEF). In addition, cell morphologies on scaffolds were analyzed with SEM. The results showed that PVA-CHI based cryogels had no genotoxic and cytotoxic effects on MEF cells and have a potential for tissue engineering applications. © 2021, Turkish Chemical Society. All rights reserved.
dc.description.sponsorshipScientific Research Projects Unit of Adana Alparslan Türkeş Science and Technology University, (BAP-20103004)
dc.identifier.doi10.18596/jotcsa.825115
dc.identifier.endpage78
dc.identifier.issn2149-0120
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85097409780
dc.identifier.scopusqualityQ3
dc.identifier.startpage69
dc.identifier.trdizinid396362
dc.identifier.urihttps://doi.org/10.18596/jotcsa.825115
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/396362
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1553
dc.identifier.volume8
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTurkish Chemical Society
dc.relation.ispartofJournal of the Turkish Chemical Society, Section A: Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectChitosan
dc.subjectCryogel
dc.subjectGenotoxicity
dc.subjectPolymeric scaffolds
dc.subjectPVA
dc.titleEvaluation of PVA/Chitosan cryogels as potential tissue engineering scaffolds; synthesis, cytotoxicity, and genotoxicity investigations
dc.typeArticle

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