Evaluation of PVA/Chitosan cryogels as potential tissue engineering scaffolds; synthesis, cytotoxicity, and genotoxicity investigations
[ X ]
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Chemical Society
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Cryogelation has become an advantageous method to obtain macro-porous materials with well-defined, interconnected pores for tissue engineering applications. Herein, polyvinyl alcohol and chitosan polymers (PVA-CHI) were used to produce cryogel scaffolds via cryogelation. Glutaraldehyde was used as a crosslinking agent and the effect of crosslinking amount on the properties of scaffolds investigated. Glutaraldehyde amount was divided into 5, 10, and 15% total amount of polymer concentration. The optimized pore morphology was obtained as a scaffold containing 5% glutaraldehyde amount. In addition to the FTIR, SEM, swelling, and degradation analyses, mechanical tests were performed to present the characterization properties of the cryogels. Direct and indirect cytotoxicity test and genotoxicity experiments were performed with Mouse Embryonic Fibroblasts (MEF). In addition, cell morphologies on scaffolds were analyzed with SEM. The results showed that PVA-CHI based cryogels had no genotoxic and cytotoxic effects on MEF cells and have a potential for tissue engineering applications. © 2021, Turkish Chemical Society. All rights reserved.
Açıklama
Anahtar Kelimeler
Chitosan, Cryogel, Genotoxicity, Polymeric scaffolds, PVA
Kaynak
Journal of the Turkish Chemical Society, Section A: Chemistry
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
8
Sayı
1
