Advancing glioblastoma research: A comparative study of drug response in novel 3D pHEMA-gelatin cryogel and traditional 2D models

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dc.authoridBaydemir Pe�int, G�zde/0000-0001-8668-8296
dc.authoridG�kt�rk, Dilek/0000-0002-1195-5828
dc.authorid�zdemir Alk��, Meryem Damla/0000-0002-5728-8004
dc.contributor.authorAlkis, Meryem Damla Ozdemir
dc.contributor.authorPesint, Gozde Baydemir
dc.contributor.authorGokturk, Dilek
dc.date.accessioned2026-02-27T07:33:11Z
dc.date.available2026-02-27T07:33:11Z
dc.date.issued2025
dc.description.abstractGlioblastoma (GBM) is the most abundant and malignant type of brain tumors. Investigating and understanding GBM biology is crucial for developing new treatments. This study aims to establish a novel 3D GBM cell culture model to investigate the drug response and comprehend GBM tumor biology. pHEMA-gelatin based cryogels are synthesized by varying cryogelation temperatures and gelatin concentrations to design a suitable scaffold. Characterization studies and in vitro cell studies show that all synthesized cryogels can be used as scaffolds for GBM, however pHEMA-gelatin-based cryogel synthesized at - 16 degrees C with 2% (w/w) gelatin concentration gave the highest cell viability test result with 221% in 3-day culture. The drug response assay is also performed by applying etoposide and temozolomide separately on GBM scaffolds by comparing 2D cultured cells. It is observed that GBM cells cultured on this cryogel exhibit greater resistance to drugs than 2D cultured cells.
dc.identifier.doi10.1557/s43578-025-01613-8
dc.identifier.endpage1820
dc.identifier.issn0884-2914
dc.identifier.issn2044-5326
dc.identifier.issue12
dc.identifier.startpage1805
dc.identifier.urihttp://dx.doi.org/10.1557/s43578-025-01613-8
dc.identifier.urihttps://hdl.handle.net/20.500.14669/4481
dc.identifier.volume40
dc.identifier.wosWOS:001507236300001
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofJournal of Materials Research
dc.relation.publicationcategoryMakale - Uluslararas� Hakemli Dergi - Kurum ��retim Eleman�
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20260302
dc.subjectBiomaterial
dc.subjectPolymer
dc.subjectBiomedical
dc.subject3D cell culture
dc.subjectPHEMA
dc.subjectCryogel
dc.titleAdvancing glioblastoma research: A comparative study of drug response in novel 3D pHEMA-gelatin cryogel and traditional 2D models
dc.typeArticle

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