Bolanthus turcicus: a promising antidiabetic with in-vitro antioxidant, enzyme inhibitory and antiadipogenic activities

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dc.authoridkoc, murat/0000-0002-0829-4571
dc.authoridDerici, Gizem Ece/0000-0002-1557-7393
dc.authoridOZDAS, Sibel/0000-0003-4610-2785
dc.authoridCanatar, Ipek/0000-0001-9448-8112
dc.contributor.authorOzdas, Sibel
dc.contributor.authorCanatar, Ipek
dc.contributor.authorDerici, Gizem Ece
dc.contributor.authorKoc, Murat
dc.date.accessioned2025-04-09T12:32:03Z
dc.date.available2025-04-09T12:32:03Z
dc.date.issued2025
dc.description.abstractIt is crucial to investigate new anti-diabetic agents and therapeutic approaches targeting molecules in potential signaling pathways for the treatment of Type 2 diabetes mellitus (T2DM). The objective of the study was to investigate the total phenolic content, antioxidant capacity, alpha-glucosidase, and alpha-amylase inhibitory activities of Bolanthus turcicus (B. turcicus), as well as their cytotoxic, anti-adipogenic, anti-diabetic, apoptotic, and anti-migration potential on adipocytes. B. turcicus samples were extracted with methanol (MeOH), ethyl acetate (EA) and aqueous (Aq) solvents. The MeOH extract had the highest phenolic content (81.14 mg GAE/g), followed by EA (74.93 mg GAE/g) and Aq (51.09 mg GAE/g). All extracts exhibited dose-dependent increases in alpha-glycosidase and alpha-amylase inhibitory activity. B. turcicus extracts showed cytotoxic effect on adipocytes with IC50 values of MeOH (141.0 mu g/mL) < Aq (155.3 g/mL) < EA (199.5 g/mL). Furthermore, B. turcicus extracts reduced lipid droplet formation and adipocyte diameter size. All extracts altered cell morphology to resemble fibroblasts. B. turcicus extracts exhibited anti-migratory effect delaying wound healing for up to 96 h. The B. turcicus extracts showed a pro-apoptotic effects on adipocytes by increasing Caspase-3 enzyme activity and the population of DAPI-positive cell with apoptotic nuclear-morphology. B. turcicus extracts upregulated the expression of the Glut-4 gene at the mRNA, protein and intracellular level in adipocytes. In conclusion, our findings indicate that B. turcicus not only exhibits strong antioxidant properties and enzyme inhibitory activities but also exerts significant anti-adipogenic and pro-apoptotic effects in adipocytes, thereby providing a comprehensive mechanism through which it may contribute to the management of T2DM. These effects highlight the potential of B. turcicus as a therapeutic agent for improving glucose homeostasis and insulin sensitivity.
dc.identifier.doi10.1007/s10735-024-10283-5
dc.identifier.issn1567-2379
dc.identifier.issn1567-2387
dc.identifier.issue1
dc.identifier.trdizinid39729235
dc.identifier.urihttp://dx.doi.org/10.1007/s10735-024-10283-5
dc.identifier.urihttps://hdl.handle.net/20.500.14669/4278
dc.identifier.volume56
dc.identifier.wosWOS:001385845300001
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Molecular Histology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20250349
dc.subjectBolanthus turcicus
dc.subjectDiabetes mellitus
dc.subjectAnti-diabetic
dc.subjectAnti-adipogenic
dc.subjectCytotoxic
dc.subjectAdipocytes
dc.titleBolanthus turcicus: a promising antidiabetic with in-vitro antioxidant, enzyme inhibitory and antiadipogenic activities
dc.typeArticle

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