Production of L-asparaginase from Candida utilis by solid-state fermentation: a comprehensive assessment of its antiproliferative potential on glioblastoma cells

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dc.authoridG�kt�rk, Dilek/0000-0002-1195-5828
dc.authorid�zdemir Alk��, Meryem Damla/0000-0002-5728-8004
dc.authoridT�lek, Ahmet/0000-0003-1079-7837
dc.authorid�PEK, SEM�H LAT�F/0000-0002-4661-7765
dc.contributor.authorAlkis, Meryem Damla Ozdemir
dc.contributor.authorIpek, Semih Latif
dc.contributor.authorTulek, Ahmet
dc.contributor.authorGunduz, Cennet Pelin Boyaci
dc.contributor.authorGokturk, Dilek
dc.date.accessioned2026-02-27T07:33:32Z
dc.date.available2026-02-27T07:33:32Z
dc.date.issued2025
dc.description.abstractL-asparaginase (L-ASNase) is an enzyme that depletes asparagine, a key amino acid for cancer cell survival, producing aspartic acid and ammonia. Beyond its food industry applications, L-ASNase is a clinically important agent against acute lymphoblastic leukemia (ALL). In this study, L-ASNase was produced and purified from Candida utilis via solid-state fermentation. Optimization on wheat bran identified 2 mL inoculation volume, 60% moisture, and a 4-day fermentation period as the optimal conditions, yielding 172.5 U/mL activity. The purified enzyme was tested against glioblastoma (GBM) cell lines, showing IC50 values of 0.4 U/mL for U87MG and 1.8 U/mL for T98G, with minimal toxicity toward normal HaCaT cells. Apoptotic effects were confirmed by DAPI/F-actin and Giemsa staining, while wound healing and clonogenic assays revealed inhibition of cell migration and colony formation. RT-qPCR analysis demonstrated downregulation of the Survivin gene, a key survival regulator. These findings highlight L-ASNase's potent antiproliferative, anti-migratory, and pro-apoptotic effects, underscoring its potential as an adjuvant therapy for GBM.
dc.description.sponsorshipAdana Alparslan Trkescedil; Science and Technology University Scientific Research Projects Unit [21103006]
dc.description.sponsorshipThis research has been supported by Adana Alparslan Turke & scedil; Science and Technology University Scientific Research Projects Unit under Grant number: 21103006.
dc.identifier.doi10.1007/s11274-025-04638-7
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue12
dc.identifier.pmid41254290
dc.identifier.urihttp://dx.doi.org/10.1007/s11274-025-04638-7
dc.identifier.urihttps://hdl.handle.net/20.500.14669/4629
dc.identifier.volume41
dc.identifier.wosWOS:001617352800001
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofWorld Journal of Microbiology & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararas� Hakemli Dergi - Kurum ��retim Eleman�
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20260302
dc.subjectL-asparaginase
dc.subjectCandida utilis
dc.subjectSolid state fermentation
dc.subjectAntiproliferative
dc.subjectGlioblastoma
dc.titleProduction of L-asparaginase from Candida utilis by solid-state fermentation: a comprehensive assessment of its antiproliferative potential on glioblastoma cells
dc.typeArticle

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