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Öğe Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models(TUBITAK, 2023) Gülnar, Birgül; Canatar, İpek; Özdaş, SibelSince obesity causes at least 2.8 million death each year and is a major risk factor for many diseases, it is critical to evaluate alternative treatment approaches. In this context, studies on the research of natural product-based therapeutics in the fight against obesity are increasing. In this study, it was aimed to evaluate the antiadipogenic and antiobesogenic efficacy of pterostilbene a natural phenolic compound in 3T3-L1 cells. The mature 3T3-L1 adipocytes were exposed to pterostilbene at different concentrations and half-maximum inhibitory concentrations (IC50 ) were determined by MTT assay. Oil-Red-O staining was applied to determine lipid accumulation. Phase contrast microscopy, Giemsa, F-actin and DAPI staining were applied to examine the efficacy of pterostilbene on the morphology of 3T3-L1 adipocyte cells. Moreover, expressions of adinopectin and glucose transporter-4 (Glut-4) in relation to insulin resistance were evaluated using immunofluorescent staining and qRT-PCR. Pterostilbene caused no significant cytotoxicity towards preadipocytes at concentrations ?7.5 ?M and the percentage of viable cells remained above about 86% for 24 h, 48 h and 72 h (p > 0.05). Therefore, pterostilbene treatment at 5 and 7.5 ?M was used in the subsequent experiments as safe dosages. In addition, it was observed that pterostilbene treatment reduced lipid accumulation in adipocyte differentiation. Adipocytes treated with a dose of 7.5 µM for 14 days showed less intense lipid deposition and a more spindle-like morphology compared to the adipocytes treated with a dose of 5 µM. Especially on the 14th day, actin filaments were filamentous in adipocytes treated with pterostilbene 7.5 µM compared to the adipocytes treated with a dose of 5 µM; the filaments were similarly oriented as in preadipocytes, and chromatin condensation was observed to be quite high. Our data suggests that the pterostilbene supplementation may help weight control and the antiadipogenic and that antiobesogenic activity is mediated in part by reduction of lipid accumulation and induction of Glut-4 and Adiponectin levels. © TÜBİTAK.Öğe Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes(Public Library of Science, 2021) Derici, Gizem Ece; Özdaş, Sibel; Canatar, Ipek; Koç, MuratDiabetes mellitus (DM) is a metabolic disorder with chronic hyperglycemia featured by metabolic outcomes owing to insufficient insulin secretion and/or insulin effect defect. It is critical to investigate new therapeutic approaches for T2DM and alternative, natural agents that target molecules in potential signal pathways. Medicinal plants are significant resources in the research of alternative new drug active ingredients. Bolanthus spergulifolius (B. spergulifolius) is one of the genera of the family Caryophyllaceae. In this study, it was explored the potential anti-diabetic effects in vitro of B. spergulifolius extracts on 3T3-L1 adipocytes. The total phenolic contents (TPC) of methanolic (MeOH), ethyl acettate (EA) and aqueous extracts of B. spergulifolius were evaluated via Folin-Ciocateau. B. spergulifolius extracts showing highly TPC (Aqueous< MeOH< EA) and their different concentrations were carried out on preadipocytes differentiated in to mature 3T3-L1 adipocytes to investigate their halfmaximal (50%) inhibitory concentration (IC50) value by using Thiazolyl blue tetrazolium bromide (MTT) assay. The IC50 of MeOH, EA and Aqueous extracts were observed as 305.7 ± 5.583 ?g/mL, 567.4 ± 3.008 ?g/mL, and 418.3 ± 4.390 ?g/mL and used for further experiments. A live/dead assay further confirmed the cytotoxic effects of MeOH, EA and Aqueous extracts (respectively, 69.75 ± 1.70%, 61.75 ± 1.70%, 70 ± 4.24%, and for all p< 0.05). Also, effects of extracts on lipid accumulation in mature 3T3-L1 adipocytes were evaluated by Oil- Red O staining assay. The extracts effectively decreased lipid-accumulation compared to untreated adipocytes (for all p< 0.05). Moreover, effect of extracts on apoptosis regulated by the Bax and Bcl-2 was investigated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The extracts significantly induced apoptosis by up-regulating pro-apoptotic Bax expression but down-regulated anti-apoptotic Bcl-2 gene expression compared to untreated adipocytes (for all p< 0.05). The Glut-4 expression linked with insulin resistance was determined by qRT-PCR, Western-blot analysis, and immunofluorescence staining. In parallel, the expression of Glut-4 in adipocytes treated with extracts was significantly higher compared to untreated adipocytes (for all p< 0.05). Extracts significantly suppressed cell migration after 30 h of wounding in a scratch-assay (for all p< 0.05). Cell morphology and diameter were further evaluated by phase-contrast microscopy, scanning electron microscopy, Immunofluorescence with F-Actin and Giemsa staining. The adipocytes treated with extracts partially lost spherical morphology and showed smaller cell-diameter compared to untreated adipocytes (for all p< 0.05). In conclusion, these results suggest that extracts of B. spergulifolius cause to an induce apoptosis, decrease lipid-accumulation, wound healing, up-regulating Glut-4 level and might contribute to reducing of insulin-resistance in DM. © 2021 Derici et al.Öğe CRM1 Geninin Baş Boyun Kanseri ve Metastazındaki Rollerinin Araştırılması(2016) Özdaş, Talih; Özdaş, SibelBirçok teknolojik gelişmelere rağmen baş boyun kanserlerinin sürvisinde önemli bir değişiklik olmamış ve insan hayatı için büyük bir tehdit oluşturmaya devam etmektedir. Baş boyun kanserlerinde başlıca tedavi yöntemi cerrahi uygulamalar olup, yüz, ağız ve laringofarinks gibi organlarda ciddi şekil bozukluklarına neden olmaktadır. Dolayısıyla bu uygulamalar hastaların yutma, konuşma gibi fonksiyonlarını da etkilemekte ve yaşam kalitesini düşürmektedir. Ayrıca kemoterapi gibi güncel tedavi yaklaşımları da toksisite gibi önemli yan etkilere sahiptir. Diğer yandan bu tedavilerin etkinliğini sorgulayan yüksek nüks ve metastaz oranları, alternatif tedavi yöntemlerine ihtiyaç olduğunu göstermektedir. Birçok açıdan baş boyun kanserleri hastada psikolojik, ekonomik ve sosyal sorunlara neden olmaktadır. Son 20-30 yıldaki insan genom teknolojisindeki ilerleme ve gelişmeler, yeni tanı, tedavi ve daha etkin koruyucu yöntemlerin gelişmesine olanak sağlamaktadır. Çoğu kanserde olduğu gibi, baş ve boyun kanseri de temel olarak genetik bağlantılı bir hastalıktır. Çeşitli genlerin kümülatif değişiklikleri baş boyun kanserinin gelişiminden sorumludur. Baş ve boyun kanserinde rol alan çeşitli genlerin tespit edilmesi, bu genlerin kanser patogenezindeki etkilerinin bilinmesi ve kanser tedavisinde kullanılabilmeleri ciddi önem arz etmektedir. Bu çalışmada da çeşitli kanser türlerinde aktif rolü olduğu kanıtlanan, tümör büyümesinde ve metastazında etkileri ortaya konulmuş, kritik bir gen olan CRM1?in baş boyun kanseri üzerine etkileri araştırılmıştır. Çalışmamız kapsamında, baş boyun kanserine ait UT-SCC-74A ve metastazı UT-SCC-74B hücre hatları kullanılmıştır. Hücreler üzerinde CRM1 geninin ekspresyonu immunofloresan boyama ile gösterilmiştir. Kültüre edilen hücrelerin transfeksiyon etkinliği GAPDH-siRNA ile belirlenmiş ve ardından hücrelere CRM1-siRNA transfekte edilerek CRM1 geni baskılanmıştır. Baskılanma düzeyleri kantitatif real time PCR ve western blot analizleriyle gösterilmiştir. CRM1 geninin baş boyun kanseri hücre hatları üzerinde proliferasyona olan etkileri xCELLigence gerçek zamanlı hücre analiz sistemi ile araştırılmıştır. Aynı zamanda proliferasyon analizlerinin validasyonu için XTT testi ve in vitro scratch assay testi yapılmıştır. Çalışmamız sonucunda UT-SCC-74A ve UT-SCC-74B hücre hatlarında CRM1 geni ekspresyonu gösterilmiştir. Hücrelerde CRM1 ekspresyonu CRM1-siRNA kullanılarak anlamlı şekilde baskılanmıştır. Optimal baskılama şartlarında gerçekleştirilen proliferasyon ve migrasyon analiz sonuçlarına göre hem UT-SCC-74A hem de UT-SCC-74B hücrelerinde CRM1 ekspresyonunun baskılanması kanser hücrelerinin büyümesini ve göç kabiliyetini de anlamlı şekilde azaltmıştır. CRM1 ekpsresyonu baskılandığında hücrelerde kaspaz 3 aktivite ölçüm sonuçlarına göre de her iki hücre grubunda da CRM1-siRNA ve kontrol grupları arasında anlamlı bir fark oluşmamıştır. Sonuç olarak; çalışmamız kapsamında CRM1 geninin baş boyun kanser ve metastazlı hücreleri üzerindeki etkileri ortaya konulmuştur. Bu fonksiyonel çalışma ile CRM1 geninin baş boyun kanserlerindeki temel moleküler rolleri belirlenmiş ve bundan sonraki aşamada mevcut agresif tedavilere alternatif olarak CRM1 geni ileyeni moleküler tedavi yöntemlerine temel oluşturulmuştur.Öğe Determination of Neopterin as a Prognostic Indicator Using Neopterin-Imprinted Cryogel Membranes(Humana Press Inc., 2021) Zenger, Okan; Eren, Burcu; Arısoy, Pırıl; Özdaş, Sibel; Baydemir Peşint, GözdeNeopterin (Neo) is thought of as a key biomarker for the diagnosis and prognosis of a wide variety of diseases associated with cellular immune response. Therefore, it has become a vital need to be able to specifically determine the Neo concentration in human serum. Molecularly imprinted cryogels have come into prominence among other affinity systems by combining advantages of Molecular Imprinting Technology (MIT) and cryogels. In this chapter, synthesis of novel Neopterin-imprinted cryogel membranes (Neo-mip), characterization studies of synthesized materials, and their use in the determination of Neo in human serum is described in detail. In addition, the evaluation of selective Neo adsorption properties of Neo-mip against competitors (Pterin and Glucose) is discussed. Neo-mip will come into prominence as important affinity materials for the selective Neo recognition in body fluids, prior to use in the health sector. © 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.Öğe Effects of Knockdown of XPO5 by siRNA on the Biological Behavior of Head and Neck Cancer Cells(John Wiley and Sons Inc, 2022) Özdaş, Sibel; Canatar, İpek; Özdaş, TalihObjectives/Hypothesis: Dysregulated expression of microRNAs (miRNAs) and dysregulation of the mechanisms that regulate them are associated with carcinogenesis. Exportin-5 (XPO5), a member of the Karyopherin family, is responsible for the transfer of pre-miRNAs from the nucleus to the cytoplasm. Despite the high oncogenic potential of XPO5 as a critical regulator of the biogenesis of miRNAs, its role in head and neck squamous cell carcinoma (HNSCC) biology has not been explained yet. Study Design: In-vitro translational. Methods: The expression of XPO5 at the mRNA, protein, and intracellular level in SCC-9, FaDu SCC-90, and Detroit-562 cell lines were evaluated with quantitative reverse transcription polymerase chain reaction, Western-blot analysis, and immunofluorescence staining, respectively. The functional role of XPO5 in HNSCC was analyzed by silencing the gene expression with XPO5-small interfering RNA (siRNA) in the in vitro model. Cell proliferation, migration capacity, and apoptosis in XPO5 knockdown HNSCC cell lines were evaluated by MTT, wound-healing, and caspase-3 assay, respectively. Results: Expression of XPO5 was determined to be upregulated at mRNA, protein, and intracellular level in metastatic cells compared to primary cells in HNSCC. XPO5 gene expression was knockdown by XPO5-siRNA transfection, verifying that it was suppressed at the mRNA, protein, and intracellular level. Silencing XPO5 caused a decrease in cell proliferation, delay in wound healing, and increase in Caspase-3 enzyme activity in HNSCC cell lines compared to control. Conclusions: This report is the first to describe the oncogenic role of XPO5 in HNSCC biology by in vitro experiments. Consequently, XPO5 can be used as a potential biomarker and therapeutic target molecule against the disease in the diagnosis-treatment-follow-up of HNSCC. Level of Evidence: NA Laryngoscope, 132:569–577, 2022. © 2021 The American Laryngological, Rhinological and Otological Society, Inc.Öğe Eksportin-1 ve P33 ING1b Proteinlerinin İlişkisinin Araştırılması(2017) Özdaş, Sibel; Özdaş, Talih-Öğe Knockdown of SET Domain, Bifurcated 1 suppresses head and neck cancer cell viability and wound-healing ability in vitro(Scientific and Technical Research Council of Turkey, 2019) Özdaş, SibelHead and neck cancer (HNC) is the sixth most common cancer worldwide and therefore presents a global public health problem. There are no standard algorithms for the diagnosis and follow-up of the disease, and no effective current treatment approaches exist. Therefore, the discovery of new biomolecules and the design of new strategies to aid in early diagnosis is necessary, along with establishing prognostic factors of HNC. In several cancer studies, the upregulation of SET Domain, Bifurcated 1 (SETDB1) has been reported to be tumor-inducing and to indicate a cancer-invasive prognosis, leading to the modulation of genes associated with different signaling pathways; however, the literature is sparse regarding the relationship between SETDB1 and HNC. In our study, three HNC primary cell lines and their corresponding metastatic cell lines were used. The quantitative reverse transcriptase-polymerase chain reaction and western blotting data indicated that the SETDB1 mRNA and protein expression levels were higher in all metastatic cell lines compared to their primary cell lines (P < 0.05 for all). To investigate the role of SETDB1 in HNC biology, in vitro functional analyses were carried out using small interference RNA (siRNA) technology, cell viability, scratch wound-healing, and the caspase-3 activity assay of gene expression of SETDB1 to compare primary and metastatic cell lines of HNC. Metastatic cells were more susceptible to this suppression, which decreased the vitality of cells and their ability of wound-healing and induced level of caspase-3 activity (P < 0.05 for all). This functional study has shown that SETDB1 plays an important role in head and neck carcinogenesis. Therefore, SETDB1 may be an attractive therapeutic target molecule and also a potential diagnostic and prognostic biomarker in HNC. © TÜBİTAK.Öğe Nazal polipozis’e eşlik eden IL5 (-746), IL6 (-174) ve IL18 (-607) gen polimorfizimleri(2021) Atilla, Mahmut Huntürk; Özdaş, Sibel; Baştimur, Sibel; Özdaş, Talih; Muz, Sami Engin; Oz, Isilay; Canatar, İpek Canatar İpekGİRİŞ ve AMAÇ: Nazal polipozis (NP), nazal mukozanın en sık karşılaşılan patolojik değişikliği olup, mukozal inflamasyon ile karakterize iyi huylu kronik bir hastalığıdır. Bu çalışmada, NP hastalarında yaygın olarak gözlenen İnterlökin (IL)5, IL6 ve IL18 gen promotor bölgesinde yer alan sırasıyla -746 C/T, -174 G/C ve -607 C/A tek nükleotidlik polimorfizmleri (SNPs) ile NP arasındaki ilişkiyi araştırmaktır. YÖNTEM ve GEREÇLER: Çalışmaya 87 NP’li hasta ve 76 kontrol olmak üzere 163 hasta dahil edilerek, polimorfizmler Snap-Shot ile genotiplendirildi. SNP'lerin bağlantı dengesizliğini değerlendirmek, allel, genotip ve haplotip frekanslarının analizi için bir lojistik regresyon modeli olan SNPStats kullanıldı. MDR ile polimorfizmlerin birbiriyle ve klinik değişkenlerle arasındaki etkileşimler değerlendirildi. BULGULAR: Çalışmamızda, IL5 ve IL18 SNP’lerinin varlığı, majör allellerinin ve CC genotiplerinin frekansı NP'de yüksekti (sırasıyla, P< 0.001ve P< 0.001; P< 0.001 ve P< 0.001; P= 0.023 ve P= 0.006). Ayrıca IL18 SNP, aspirin intoleransı ve astımatik NP’lilerde daha sık gözlendi (P= 0.013 ve P= 0.045). Bununla birlikte IL5-IL6-IL18 CGC haplotipinin frekansı NP’li hasta grubunda yüksekti (P< 0.0001). MDR analizi ile tespit edilen en iyi tek-lokus modeline göre IL18 CC genotipinin, iki-lokus modeline göre IL5_IL18’in majör allelli içeren diplotiplerinin artmış-NP riski ile ilişkili olduğu bulundu (P= 0.006, P< 0.0001). IL5 genotiplerinin ve IL5_IL6 diplotiplerininin NP'de risk paterni IL18’ün genotipine bağlı olduğu gözlendi (P< 0.0001). Ayrıca NP-pozitif aile öyküsüne sahip bireylerin 16-kat artmış NP riski taşıdığı gözlenmiştir (P= 0.0004). TARTIŞMA ve SONUÇ: Sonuç olarak, çalışmamız IL5 (-746) ve IL18 (-607) polimorfizmlerinin nazal polipozis için predispozan faktörler olduğunu göstermiştir. Nazal polipoziste IL5 (-746) ve IL18 (-607) SNP’lerinin gen aktivitesi üzerindeki sonuçlarını araştıran ileri fonksiyonel çalışmalara ihtiyaç vardır.Öğe Neopterin-Imprinted Columns for Selective Neopterin Recognition from Serum and Urine Samples(Elsevier Ltd, 2021) Özdaş, Sibel; Baydemir Peşint, Gözde; Arısoy, Pırıl; Zenger, Okan; Eren, BurcuNeopterin (Np), a catabolic product of guanosine triphosphate (GTP), is synthesized by human macrophages and is an important indicator of cellular immune system activation. Np is associated with the activated cellular immune response and therefore this molecule is a potential biomarker for the diagnosis and follow-up of a wide variety of physiological conditions including cardiovascular and neurodegenerative diseases, autoimmune disorders and viral infections. Within the scope of this study, it was aimed to synthesize neopterin-imprinted cryogel columns (Np-MIPs), which can selectively recognize Np in human body fluids. Np-MIPs were synthesized via the cryo-polymerization in presence of Np as the template molecule and characterized by swelling test, polymerization yield calculations, Brunauer-Emmett-Teller (BET) measurements and Scanning Electron Microscopy (SEM). The surface area was measured as 22 m2/g. Adsorption and desorption of Np from aqueous solutions were investigated, and selectivity studies were performed against pterine and glucose molecules. Maximum Np adsorption on Np-MIP was found to be 249.2 ?g/g and Np-MIPs can adsorb Np 1.09 and 3.84 times selective than glucose and pterin, respectively. It was demonstrated that Np-MIP columns can selectively adsorb Np from serum and urine, with the adsorption capacities of 36 ?g/g and 38.2 ?g/g Np-MIP, respectively. © 2021Öğe Phyllanthus emblica-Loaded Cryogels for Improved Wound Care: Characterization and In Vitro Studies(John Wiley and Sons Inc, 2024) Canatar, İpek; Özdaş, Sibel; Baydemir Peşint, GözdeWound dressings developed by combining plant extracts with polymers have made a great progress in wound care treatment. One plant with remarkable healing properties is Phyllanthus emblica Linn (P. emblica), which is described as having potent antioxidant, antimicrobial and anti-inflammatory properties. The aim of this study is to evaluate the biocompatibility of P. emblica-loaded polyvinyl alcohol/gelatin-based cryogels (PVA/Gel/P.emblica) through cytotoxicity and proliferation tests in HaCaT cells and examine their potential in wound dressing applications. Accordingly, PVA/Gel/P.emblica cryogels are successfully synthesized and characterization studies and in vitro cell culture studies are performed. The swelling tests and Brunauer–Emmett–Teller analysis results show that swelling and surface area properties of cryogels increase with increasing P. emblica amounts. Morphological results display that the cryogels have a dense, interconnected pore morphology and a macroporous structure. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, trypan blue exclusion, and live–dead assay results reveal that P. emblica enhances cell proliferation, increases cell number, and improves cell viability. Based on the scanning electron microscope, immunofluorescence, and Giemsa staining images, it is observed that P. emblica promotes cell attachment, proliferation, and penetration. These findings confirm that PVA/Gel/P.emblica cryogels are suitable for use as wound dressing materials and can be developed with further studies. © 2024 The Authors. Macromolecular Materials and Engineering published by Wiley-VCH GmbH.Öğe Protective Effect of 5-HTTLPR (S) and VNTR (10) Allele Combinationsof5-HTT Gene Against Adenotonsillary Hypertrophy(2021) Muz, Sami Engin; Özdaş, Sibel; Özdaş, Talih; Atilla, Mahmut Huntürk; Baştimur, Sibel; Öz, Işılay; Canatar, İpekINTRODUCTION: Serotonin transporter protein which is coded by 5HTT gene is responsible forpresynaptic reuptake of serotonin. In this study, we investigated the relationship between polymorphismsin the promoter region (5-HTTLPR) and in the second intron (VNTR) in the 5-HTTgene and adenotonsillarhypertrophy (ATH) in pediatric cases. METHODS: Genotyped of the 5-HTT gene promotor 5-HTTLPR and intronic VNTR of in 197 childrenwere analyzed using Snap Shot, Multifactor Dimensionality Reduction (MDR) software and carried out toassess the interactions among two polymorphisms and phenotype. RESULTS: A total of 119 children with ATH (48 girls, 71 boys age range: 3-10 years; mean age: 5.38years) and 78 healthy children (27 girls, 51 boys, age range: 4-13 years; mean age: 6.76 years) wereincluded in this study. The frequencies of the genotype in all of inheritance models of the 5-HTTLPR andthe VNTR (10) allele showed no significant differences between ATH patient and healthy controls (for allP> 0.05). However, frequency of the 5-HTTLPR (S) allele and VNTR_5-HTTLPR (10/S) haplotype and(10/10+S/S) diplotype were significantly higher in the control group compared to ATH cases (P= 0.048,P= 0.041, P= 0.13). DISCUSSION AND CONCLUSION: In this study, we observed that S/S genotype, 10/S haplotype and10/10 + S/S diplotype of 5-HTT gene could have protective effect against ATH.Öğe Pterostilbene loaded poly(vinyl alcohol)-gelatin cryogels as potential bioactive wound dressing material(John Wiley and Sons Inc, 2023) Canatar, İpek; Zenger, Okan; Özdaş, Sibel; Baydemir Peşint, GözdeCryogels are support materials which are good at mimicking extracellular matrix due to their excellent hydrophilicity, biocompatibility, and macroporous structure, thus they are useful in facilitating cell activities during healing process. In this study, polyvinyl alcohol-gelatin (PVA-Gel) based cryogel membranes loaded with pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene; PTS) (PVA-Gel/PTS) was synthesized as wound dressing materials. PVA-Gel and PVA-Gel/PTS were synthesized with the polymerization yields of 96% ± 0.23% and 98% ± 0.18%, respectively, and characterized by swelling tests, Brunauer–Emmett–Teller (BET) and scanning electron microscopy (SEM) analysis. The swelling ratios were calculated as 98.6% ± 4.93% and 102% ± 5.1%, macroporosities were determined as 85% ± 2.13% and 88% ± 2.2%, for PVA-Gel and PVA-Gel/PTS, respectively. It was determined that PVA-Gel and PVA-Gel/PTS have 17 m2/g ± 0.76 m2/g and 20 m2/g ± 0.92 m2/g surface areas, respectively. SEM studies were demonstrated that they have ~100 ?m pore sizes. According to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue exclusion and live-dead assay results, it was observed that cell proliferation, cell number and cell viability were higher in PVA-Gel/PTS cryogel at 24, 48, and 72 h compared to PVA-Gel. A strong and transparent fluorescent light intensity was observed indicating higher cell population in PVA-Gel/PTS in comparison with PVA-Gel, according to 4?,6-diamidino-2-phenylindole (DAPI) staining. SEM, F-Actin, Giemsa staining and inverted-phase microscope image of fibroblasts in PVA-Gel/PTS cryogels revealed that dense fibroblast proliferation and spindle-shaped morphology of cells were preserved. Moreover, DNA agarose gel data demonstrated that PVA-Gel/PTS cryogels had no effect on DNA integrity. Consequently, produced PVA-Gel/PTS cryogel can be used as wound dressing material to promote wound therapies, inducing cell viability and proliferation. © 2023 Wiley Periodicals LLC.Öğe The common genetic variants of toll-like receptor and susceptibilityto adenoid hypertrophy: a hospital-based cohort study(2016) Babademez, Mehmet Ali; Özdaş, Talih; Özdaş, SibelBackground/aim: Adenoid hypertrophy (AH) is one of the most frequent pediatric disorders. The aim of this study was to investigate the effects of TLR2-R753Q, TLR4-T399I, and TLR4-D299G polymorphisms in children with AH. Materials and methods: The variants of the TLR gene were determined by restriction fragment length polymorphism (PCR-RFLP) analysis in 60 patients with AH and in 50 healthy children. Data were analyzed with SNPStats and multifactor dimensionality reduction (MDR) software. Results: We found that the presence of the G allele, the AG+GG and AG genotypes at TLR4-D299G, and the GGT haplotype were associated with AH in children (P = 0.013, P = 0.02, P = 0.038, and P = 0.001, respectively). On the contrary, no association was found between TLR2-R753Q and predisposition to AH. The CT genotype at TLR4-T399I showed a sex-specific association with AH, occurring only in boys with allergies (P = 0.0048). In addition, MDR analysis indicated a strong synergy between TLR gene markers contributing to AH. Allergic children with the diplotypes that included minor alleles of TLR4-D299G or TLR4-T399I had about a 4-fold increased risk for AH. Conclusion: Common genetic variants of the gene encoding the TLR4 protein may have differential effects on AH and the presence of sex-specific allergy.
