Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor

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dc.contributor.authorKibar, Güneş
dc.contributor.authorŞahinoğlu, O. Berkay
dc.contributor.authorKılınçlı, Betül
dc.contributor.authorErdem, E. Yegan
dc.contributor.authorÇetin, Barbaros
dc.contributor.authorÖzalp, V. Cengiz
dc.date.accessioned2025-01-06T17:29:59Z
dc.date.available2025-01-06T17:29:59Z
dc.date.issued2024
dc.description.abstractThis study introduces aptamer-functionalized polyhedral oligomeric silsesquioxane (POSS) nanoparticles for adenosine triphosphate (ATP) detection where the POSS nanoparticles were synthesized in a one-step, continuous flow microfluidic reactor utilizing thermal polymerization. A microemulsion containing POSS monomers was generated in the microfluidic reactor which was designed to prevent clogging by using a continuous oil flow around the emulsion during thermal polymerization. Surfaces of POSS nanoparticles were biomimetically modified by polydopamine. The aptamer sequence for ATP was successfully attached to POSS nanoparticles. The aptamer-modified POSS nanoparticles were tested for affinity-based biosensor applications using ATP as a model molecule. The nanoparticles were able to capture ATP molecules successfully with an affinity constant of 46.5 ?M. Based on this result, it was shown, for the first time, that microfluidic synthesis of POSS nanoparticles can be utilized in designing aptamer-functionalized nanosystems for biosensor applications. The integration of POSS in biosensing technologies not only exemplifies the versatility and efficacy of these nanoparticles but also marks a significant contribution to the field of biorecognition and sample preparation. © The Author(s) 2024.
dc.description.sponsorshipAdana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi, ATÜ, (22103005); Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (122C228, TÜBİTAK-2218)
dc.identifier.doi10.1007/s00604-024-06186-7
dc.identifier.issn0026-3672
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85185673644
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-024-06186-7
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1439
dc.identifier.volume191
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectAptasensor
dc.subjectATP detection
dc.subjectBiosensor
dc.subjectMicrofluidics
dc.subjectPolyhedral oligomeric silsesquioxane (POSS)
dc.titleBiosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor
dc.typeArticle

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