Neopterin-Imprinted Columns for Selective Neopterin Recognition from Serum and Urine Samples

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dc.contributor.authorÖzdaş, Sibel
dc.contributor.authorBaydemir Peşint, Gözde
dc.contributor.authorArısoy, Pırıl
dc.contributor.authorZenger, Okan
dc.contributor.authorEren, Burcu
dc.date.accessioned2025-01-06T17:30:10Z
dc.date.available2025-01-06T17:30:10Z
dc.date.issued2021
dc.description.abstractNeopterin (Np), a catabolic product of guanosine triphosphate (GTP), is synthesized by human macrophages and is an important indicator of cellular immune system activation. Np is associated with the activated cellular immune response and therefore this molecule is a potential biomarker for the diagnosis and follow-up of a wide variety of physiological conditions including cardiovascular and neurodegenerative diseases, autoimmune disorders and viral infections. Within the scope of this study, it was aimed to synthesize neopterin-imprinted cryogel columns (Np-MIPs), which can selectively recognize Np in human body fluids. Np-MIPs were synthesized via the cryo-polymerization in presence of Np as the template molecule and characterized by swelling test, polymerization yield calculations, Brunauer-Emmett-Teller (BET) measurements and Scanning Electron Microscopy (SEM). The surface area was measured as 22 m2/g. Adsorption and desorption of Np from aqueous solutions were investigated, and selectivity studies were performed against pterine and glucose molecules. Maximum Np adsorption on Np-MIP was found to be 249.2 ?g/g and Np-MIPs can adsorb Np 1.09 and 3.84 times selective than glucose and pterin, respectively. It was demonstrated that Np-MIP columns can selectively adsorb Np from serum and urine, with the adsorption capacities of 36 ?g/g and 38.2 ?g/g Np-MIP, respectively. © 2021
dc.description.sponsorshipNanotechnology Research and Application Laboratory in Adana Alparslan Türkeş Science and Technology University; Scientific Research Projects Coordination Unit of Adana Alparslan Türkeş Science and Technology University, (18103018); Çukurova Development Agengy/Turkey, (TR62/18ÜRET/0032)
dc.identifier.doi10.1016/j.procbio.2021.05.022
dc.identifier.endpage7
dc.identifier.issn1359-5113
dc.identifier.scopus2-s2.0-85107284563
dc.identifier.scopusqualityQ1
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2021.05.022
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1493
dc.identifier.volume108
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241211
dc.subjectBiomarker determination
dc.subjectCryogels
dc.subjectMolecular imprinting
dc.subjectNeopterin
dc.titleNeopterin-Imprinted Columns for Selective Neopterin Recognition from Serum and Urine Samples
dc.typeArticle

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